医学分子生物学杂志2019，Vol.16Issue(2)：158-163,6.DOI:10.3870/j.issn.1672-8009.2019.02.010
松果菊苷促进卵巢癌模型裸鼠存活、降低肿瘤增殖和转移
Echinacoside Promotes the Survival of Nude Mouse Models of Human Ovarian Cancer and Inhibits the Proliferation and Metastasis of Tumors
摘要
Abstract
Objective To investigate the effects of echinacoside ( ES) on the survival of the mouse model of human ovarian cancer, and the growth and metastasis of tumors.Methods Sixty nude mice were randomly divided into four groups.In the control group, the mice were subcutane-ously injected with ovarian cancer CoC1 cells that could stably express luciferase and then treated with equal amount of saline.In ES groups ( 20, 50, 100 mg/kg) , different doses of ES ( 20, 50, 100 mg/kg) were intraperitoneally given to the mouse model of ovarian cancer.The treatment lasted for 25 days.The survival and tumor volume of nude mice were recorded every day.The mice were sacrificed at 26 days.Tumor tissues were harvested and immunohistochemically detected for the expression levels of Ki67 and vascular endothelial growth factor ( VEGF) .TUNEL was used to de-tect apoptosis.Results Compared with the control group, the survival rate of ES (20, 50, 100 mg/kg) groups was significantly increased (P<0.05), the tumor volume significantly decreased, and the Ki67 positive cells in the transplanted tumor tissues significantly decreased ( P <0.05) . .The number of apoptotic cells in the ES (50, 100 mg/kg) groups was significantly higher than that in the control group ( P <0.05) .The number of VEGF positive cells in the ES (20, 50, 100 mg/kg) group was significantly lower than that in the control group (P<0.05) .In addition, the fluorescence intensity of the heart, pancreas, liver and kidney of the ES (50, 100 mg/kg) groups was significantly lower than that of the control group.Conclusion ES promotes the survival of the mouse model of ovarian cancer, and inhibits the growth and metastasis of ovarian cancer.关键词
松果菊苷/ 卵巢癌/ 肿瘤生长/ 迁移Key words
echinacoside/ ovarian cancer/ tumor growth/ metastasis分类
医药卫生引用本文复制引用
郑若梅,夏良斌,汪晶,曹阳,王滟..松果菊苷促进卵巢癌模型裸鼠存活、降低肿瘤增殖和转移[J].医学分子生物学杂志,2019,16(2):158-163,6.基金项目
湖北省卫生厅科研基金 (No.JX6 C-25) This work was supported by a grant from the Scientific Research Fund of Hubei Provincial Health Department (No.JX6 C-25) （No.JX6 C-25）
