神经损伤与功能重建2019，Vol.14Issue(3)：109-112,4.DOI:10.16780/j.cnki.sjssgncj.2019.03.001
星形胶质细胞特异性敲除Adam10基因对周细胞和血脑屏障的影响
Effects of Knockout Astrocytic Adam10 Gene on Pericytes and B1ood Brain Barrier
摘要
Abstract
Objective: To investigate the effect of Adam10 gene on the cross-ta1k between astrocyte and peri-cyte, and on the integrity of b1ood-brain barrier (BBB). Methods: Adam10 gene knockout mice (Gfapcre-Ad-am101ox/1ox) were obtained by Cre-1oxp technique. Adam101ox/1ox mice were used as contro1. The expression of myo-cardin and smooth muscu1ar actin (SMA) protein in pericytes was detected by doub1e immunof1uorescence 1abe1-ing technique. The degeneration and apoptosis of pericytes, destruction of basement membrane and BBB were an-a1yzed by e1ectron microscopy. Laser speck1e imaging was used to observe the changes of regiona1 cerebra1 b1ood perfusion. Resu1ts: Specific knockout of Adam10 gene in astrocytes affectted the signa1 exchange between astro-cyte and pericyte; decreased the myocardin and SMA protein expression in pericytes; induced pericyte degenera-tion and apoptosis; damaged BBB; and affected vascu1ar density and b1ood f1ow ve1ocity in brain cortex. Conc1u. sion: Adam10 gene may affect the signa1 exchange between astrocytes and pericytes, thus affecting the integrity of BBB and cerebra1 b1ood perfusion.关键词
星形胶质细胞/周细胞/Adam10/血脑屏障/激光散斑衬比成像Key words
astrocyte/ pericyte/ Adam10/ b1ood brain barrier/ 1aser speck1e imaging分类
医药卫生引用本文复制引用
熊颖,张强,潘邓记..星形胶质细胞特异性敲除Adam10基因对周细胞和血脑屏障的影响[J].神经损伤与功能重建,2019,14(3):109-112,4.基金项目
国家自然科学基金(No. 81471230 （）
No. 81601480 （）
No. 81571206)基金项目 （）
