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烟草KNOX基因家族的结构与表达模式分析

刘向真 杨永锋 王中 王根发 金立锋 张林 魏攀 王晨 李泽锋 王宇博 王燃

烟草科技2019,Vol.52Issue(2):1-11,11.
烟草科技2019,Vol.52Issue(2):1-11,11.

烟草KNOX基因家族的结构与表达模式分析

Structure and expression analysis of KNOX gene family in tobacco

刘向真 1杨永锋 1王中 2王根发 1金立锋 2张林 3魏攀 2王晨 2李泽锋 2王宇博 4王燃2

作者信息

  • 1. 河南中烟工业有限责任公司技术中心,郑州经济技术开发区第三大街9号 450000
  • 2. 中国烟草总公司郑州烟草研究院,郑州高新技术产业开发区枫杨街2号 450001
  • 3. 河南农业大学烟草学院,郑州市金水区农业路63号 450002
  • 4. 郑州大学生命科学学院,郑州高新技术产业开发区科学大道100号 450001
  • 折叠

摘要

Abstract

KNOX genes encode transcription factors with homeobox domains, which play important roles in plant morphogenesis and pattern formation. To explore the structure and functions of KNOX genes from tobacco, 18, 8 and 5 KNOX genes were identified from Nicotiana tabacum, N. sylvestris and N. tomentosiformis, respectively. In addition, their phylogenetic, gene structure, protein functional domains and gene expression patterns were analyzed. The results showed that KNOX genes from tobacco could be divided into two subclasses:NtKNOX Ⅰ and NtKNOX Ⅱ, and the two subclasses were further divided into 5 evolutionary branches. In the same evolutionary branch, the functional domains of tobacco KNOX proteins and their coding sequences were highly conserved in gene structure. Expression analysis indicated that NtKNOX Ⅱ genes in N. tabacum were expressed in multiple tissues, and the overall expression level was higher than that of NtKNOX Ⅰgenes. The high expressed genes were different in roots and leaves of N. sylvestris and N. tomentosiformis.

关键词

烟草/KNOX基因家族/基因结构/基因表达/功能域

Key words

Tobacco/KNOX gene family/Gene structure/Gene expression/Functional domain

分类

轻工纺织

引用本文复制引用

刘向真,杨永锋,王中,王根发,金立锋,张林,魏攀,王晨,李泽锋,王宇博,王燃..烟草KNOX基因家族的结构与表达模式分析[J].烟草科技,2019,52(2):1-11,11.

基金项目

湖南省烟草专卖局科技项目"湘西烟区土壤pH变化机制及调控技术研究与应用"(xx15-18Aa01). (xx15-18Aa01)

烟草科技

OA北大核心CSCDCSTPCD

1002-0861

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