烟草科技2019,Vol.52Issue(2):1-11,11.
烟草KNOX基因家族的结构与表达模式分析
Structure and expression analysis of KNOX gene family in tobacco
摘要
Abstract
KNOX genes encode transcription factors with homeobox domains, which play important roles in plant morphogenesis and pattern formation. To explore the structure and functions of KNOX genes from tobacco, 18, 8 and 5 KNOX genes were identified from Nicotiana tabacum, N. sylvestris and N. tomentosiformis, respectively. In addition, their phylogenetic, gene structure, protein functional domains and gene expression patterns were analyzed. The results showed that KNOX genes from tobacco could be divided into two subclasses:NtKNOX Ⅰ and NtKNOX Ⅱ, and the two subclasses were further divided into 5 evolutionary branches. In the same evolutionary branch, the functional domains of tobacco KNOX proteins and their coding sequences were highly conserved in gene structure. Expression analysis indicated that NtKNOX Ⅱ genes in N. tabacum were expressed in multiple tissues, and the overall expression level was higher than that of NtKNOX Ⅰgenes. The high expressed genes were different in roots and leaves of N. sylvestris and N. tomentosiformis.关键词
烟草/KNOX基因家族/基因结构/基因表达/功能域Key words
Tobacco/KNOX gene family/Gene structure/Gene expression/Functional domain分类
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刘向真,杨永锋,王中,王根发,金立锋,张林,魏攀,王晨,李泽锋,王宇博,王燃..烟草KNOX基因家族的结构与表达模式分析[J].烟草科技,2019,52(2):1-11,11.基金项目
湖南省烟草专卖局科技项目"湘西烟区土壤pH变化机制及调控技术研究与应用"(xx15-18Aa01). (xx15-18Aa01)