烟草科技2019，Vol.52Issue(2)：19-24,6.
烟草野火病菌噬菌体的分离及盆栽防治试验
Isolation of bacteriophages from Pseudomonas syringae pv. tabaci and their control efficiency against tobacco wildfire disease in pot experiments
摘要
Abstract
To control tobacco wildfire disease in fields, bacteriophages (T-109 and T-ZD) were isolated and purified from Pseudomonas syringae pv. tabaci 109 and ZD respectively. The control effects of T-109 and T-ZD on tobacco wildfire disease were analyzed by needle puncturing inoculation and spray inoculation in pot experiments. The results showed that: 1) In needle puncturing inoculation, the control efficiencies of spraying no-diluted T-109 or T-ZD first and then inoculating P. syringae pv. tabaci 109 or ZD on P. syringae pv. tabaci reached 98.64% and 96.68% , respectively. The treatments of spraying 10-fold diluted T-109 or T-ZD first and then inoculating P. syringae pv. tabaci 109 or ZD showed a control efficiency of 97.18% and 95.12% , respectively, and those of inoculating P. syringae pv. tabaci 109 or ZD first and then spraying no-diluted T-109 or T-ZD were at 63.26% and 8.47% , respectively. The treatments of inoculating P. syringae pv. tabaci 109 or ZD first and then spraying 10-fold diluted T-109 or T-ZD had an efficiency of 29.41% and 5.42%, respectively. The treatments of spraying bacteriophages first and then inoculating P. syringae pv. tabaci were better than those of inoculating P. syringae pv. tabaci first and then spraying bacteriophages. 2) In spraying inoculation, the control efficiencies of spraying no-diluted T-109 or T-ZD first and then inoculating P. syringae pv. tabaci 109 or ZD on P. syringae pv. tabaci were 27.32% and 39.44% , respectively. The treatment efficiency of spraying 10-fold diluted T-109 or T-ZD first and then inoculating P. syringae pv. tabaci 109 or ZD were -2.69% and 56.05% , respectively. The treatment efficiency of inoculating P. syringae pv. tabaci 109 or ZD first and then spraying no-diluted T-109 or T-ZD were 0.44% and 36.06% , respectively. The treatments of inoculating P. syringae pv. tabaci 109 or ZD first and then spraying 10-fold diluted T-109 or T-ZD were 28.23% and 23.79%, respectively. No significant difference was found in the control efficiencies between the treatments of spraying bacteriophages first and then inoculating P. syringae pv. tabaci and the treatments of inoculating P. syringae pv. tabaci first and then spraying bacteriophages.关键词
烟草/野火病/噬菌体/防效Key words
Tobacco/Wildfire disease/Bacteriophage/Control efficiency分类
农业科技引用本文复制引用
孙宏伟,李慧,刘瑞清,张民,荆瑞勇,刘俊杰,孙剑萍..烟草野火病菌噬菌体的分离及盆栽防治试验[J].烟草科技,2019,52(2):19-24,6.基金项目
中国烟草总公司重大专项项目"细支卷烟辅助材料系统化设计及应用技术研究"[110201601013(XZ04)] （XZ04）
河南中烟工业有限责任公司重点科技项目"细支滤棒专用丝束开发及应用研究"(YN2016005) （YN2016005）
河南中烟工业有限责任公司重点科技项目"辅材参数对细支烟烟气主要化学成分及感官影响研究"(ZW2015020). （ZW2015020）
