北京林业大学学报2018，Vol.40Issue(12)：49-59,11.DOI:10.13332/j.1000-1522.20180332
胡杨幼苗生长相关性状QTL上位性分析
QTL epistasis effect analysis of seedling growth-related traits in Populus euphratica
摘要
Abstract
[Objective] Seedling stage plays a vital role in plant growth and development. Up to date there are no reports about genetic mechanism of epistasis interaction during seedling growth in Populus euphratica. [Method]In this study, a full-sib F1 population including 408 individuals was derived from Populus euphratica × Populus euphratica. Dynamic growth data of four phenotypes were measured, including the taproot length, shoot length, total lateral root length, and number of lateral roots. Based on a high-density genetic linkage map, 2 HIGWAS was employed to identify QTL-QTL interactions associated with four phenotypes. [Result] Finally, a total of 83 pairs of QTL-QTL interactions were detected, including 83 SNPs. The taproot length, shoot length, total lateral root length and number of lateral roots found 24 pairs, 20 pairs, 24 pairs and 15 pairs epistatic interactions, respectively, and the epistatic interactions of main root length, stem height and lateral root length were distributed in linkage groups 1, 19, and 17, respectively. In addition, we conducted functional annotations for the SNPs with significant QTL-QTL epistatic interactions, and 19 QTLs were annotated with candidate genes. [Conclusion]Interactions associated with total lateral root length had higher heritability, and most of the SNPs werelocated in linkage group 17, which might be important candidate gene regions. This work may provide guidance for molecular marker-assisted breeding of Populus euphratica and other forest trees.关键词
QTL/上位效应/2HIGWAS/幼苗生长/胡杨Key words
QTL/epistasis effect/2HIGWAS/seedling growth/Populus euphratica分类
农业科技引用本文复制引用
王平,王东洋,王晶,姜立波,邬荣领..胡杨幼苗生长相关性状QTL上位性分析[J].北京林业大学学报,2018,40(12):49-59,11.基金项目
国家自然科学基金项目(31700576) （31700576）
国家林业公益性行业科研专项(201404102) （201404102）
