北京林业大学学报2018，Vol.40Issue(12)：68-75,8.DOI:10.13332/j.1000-1522.20180230
粉红粘帚霉SWFUYHL 02-03侵染楚雄腮扁叶蜂幼虫的扫描电镜及透射电镜观察
SEM and TEM observations of Clonostachys rosea SWFUYHL 02-03 infecting the body wall of Cephalcia chuxiongica larvae
摘要
Abstract
[Objective]This paper aims to study the process of Clonostachys rosea infecting Cephalcia chuxiongica larvae, and provides a reference for the research on pathogenicity mechanism of Ce.chuxiongica. [Method]We observed the ultrastructural changes of Ce. chuxiongica body wall infected by Cl. rosea SWFUYHL 02-03 using the scanning electronic microscopy (SEM) and transmission electron microscopy (TEM) . [Result] Based on the SEM observation, the conidias adhered onto spiracles and internode membranes of body wall after 6 hours inoculation. The germ tubes of this fungi penetrated into the cuticle of Ce. chuxiongica larvae after 14 hours. The conidia and hypha penetratedinto the cuticle of Ce. chuxiongica larvae and the hypha began to multiply rapidly after 26 hours, and then the hypha penetrated out from the cuticle of Ce. chuxiongica larvae and generated sporogenous structure when it was inoculated at 72 hours. Meanwhile, inferred from the TEM observation, the participation of enzymes and histolysis could be found with the penetration process of hypha. The spores adhered onto the body wall of the Ce. chuxiongica larvae when it was inoculated at 6 hours and then the conidia germinated and penetrated into the secretion of cuticle after inoculation for 14 hours. The hypha penetrated and spreaded into the cuticle, and halos with low electron density could be observed around the penetrated hypha after 26 hours. The epidermis cell arranged loose and became vacuole after inoculation for 38 hours and the larvae's body was full of hypha, and a great deal of hypha were on the cuticle of Ce. Chuxiongica larvae after inoculation for 48 hours. [Conclusion] The spores and hypha of Cl. Rosea SWFUYHL 02-03 could parasitize and kill the Ce. Chuxiongica larvae. The SEM and TEM observations accurately reflected the infection process of Cl. Rosea SWFUYHL 02-03 on the larvae of Ce.chuxiongica, and proved the existence of enzymes and virulent metabolites.关键词
粉红粘帚霉SWFUYHL 02-03/楚雄腮扁叶蜂/侵染过程/扫描电镜/透射电镜Key words
Clonostachys rosea SWFUYHL 02-03/Cephalcia chuxiongica/infection process/scanning electron microscopy (SEM)/transmission electron microscopy (TEM)分类
农业科技引用本文复制引用
芦俊佳,徐荣,李永和..粉红粘帚霉SWFUYHL 02-03侵染楚雄腮扁叶蜂幼虫的扫描电镜及透射电镜观察[J].北京林业大学学报,2018,40(12):68-75,8.基金项目
国家自然科学基金项目(31360156) （31360156）
西南林业大学云南省省级重点学科(林学)建设项目 （林学）
国家级省级大学省创新创业训练计划项目(516027) （516027）
