动物医学进展2019，Vol.40Issue(1)：1-6,6.
O型口蹄疫病毒样颗粒编码基因的真核表达载体构建及表达
Construction and Expression of Eukaryotic Expression Vector of O Type Foot-and-mouth Disease Virus-like Particles Encoding Gene
摘要
Abstract
To construct and express the eukaryotic expression vector of serotype O foot-and-mouth disease virus-like particles encoding gene, VP0, VP1 and VP3genes were cloned into the eukaryotic expression vector pVAX1 through the known FMDV structural protein gene sequences in this paper.Then, the recombinant plasmids pVAX-VP0, pVAX-VP1, pVAX-VP3 were amplified by using primers with BsmB Ⅰ, EcoR Ⅰ and Sal Ⅰ restriction sites respectively, the recombinant eukaryotic expression vector pVAXVP0VP1VP3 was obtained by ligating with T4 ligase.The recombinant eukaryotic expression vector pVAX-VP0VP1VP3 was identified by PCR and sent to Suzhou Jinzhizhi Biotechnology Co., Ltd.for sequencing.The recombinant eukaryotic expression vector pVAX-VP0VP1VP3 was transfected into BHK cells by lipofectin transfection method and the expression of recombinant eukaryotic expression vector pVAX-VP0VP1VP3 in cells was detected by indirect immunofluorescence assay test (IFAT) and Western blot.The results showed that the recombinant eukaryotic expression vector pVAX-VP0VP1VP3 was constructed and expressed successfully in BHK-21 cells.关键词
口蹄疫病毒/重组真核表达载体/pVAX-VP0VP1VP3构建和表达/病毒样颗粒Key words
Foot-and-mouth disease virus/recombinant eukaryotic expression vector/construction and expression of pVAX-VP0VP1VP3/virus-like particle分类
农业科技引用本文复制引用
李淑萍,孙世琪,莫亚霞,胡永浩,郭慧琛..O型口蹄疫病毒样颗粒编码基因的真核表达载体构建及表达[J].动物医学进展,2019,40(1):1-6,6.基金项目
动物重大疫病新概念防控产品研发项目(2017YFD0501100) （2017YFD0501100）
牛羊重要疫病免疫防控新技术研究项目(2017YFD0500900) （2017YFD0500900）
