动物医学进展2019，Vol.40Issue(1)：25-30,6.
羊源D型多杀性巴氏杆菌的分离鉴定及毒力基因检测
Isolation and Identification of Pasteurella multocida Serotype D and Detection of Virulence Genes from Sheep
摘要
Abstract
In order to determine the cause of the sheep died of respiratory infection from a sheep farm in Shihezi, bacterial pathogens were isolated and identified by conventional methods and molecular biological methods based on 16 SrRNA special sequence and Pm specific gene kmt sequence analysis.Serotype of the isolate was determined with the primers from 5capsular serotype-specific genes, 16virulence-associated genes were detected, pathogenicity and drug sensitivity of the isolate were analysed.The results showed that the isolate was a serotype D Pm with strong pathogenicity, which carried 8virulence-associated genes and the amplified fragment homology of the corresponding sequence in NCBI is more than 99％.The isolate was resistant to penicillin, lincomycin, gentamicin, cotrimoxazole.关键词
羊呼吸道疾病/D型多杀性巴氏杆菌/分离鉴定/毒力基因Key words
sheep respiratory disease/Pasteurella multocida serotype D/isolation and identification/virulence gene分类
农业科技引用本文复制引用
王蕾,钟发刚,邬琴,柴迎锦,梅朱园,郭强强,黄新,韩猛立,张星星,周霞..羊源D型多杀性巴氏杆菌的分离鉴定及毒力基因检测[J].动物医学进展,2019,40(1):25-30,6.基金项目
省部共建绵羊遗传改良与健康养殖国家重点实验室优秀中青年人才培养引导计划专项资助项目(SKLGIH2016A02) （SKLGIH2016A02）
2017年度省部共建绵羊遗传改良与健康养殖国家重点实验室开放课题(MYSKLKF 201804) （MYSKLKF 201804）
2017年国家级创新创业训练计划项目(2017107590) （2017107590）
兵团现代农业科技攻关与成果转化计划项目(2016AC018) （2016AC018）
