动物医学进展2019，Vol.40Issue(1)：30-35,6.
狐和貉源大肠埃希菌药敏试验及耐药基因检测
Drug Sensitivity Test and Drug Resistance Gene Detection of Escherichia coli from Foxes and Raccoon Dogs
摘要
Abstract
To investigate the drug resistance and resistance genes in pathogenic Escherichia coli strains isolated from foxes and raccoon dogs in Changli county of Hebei province, 15 pathogenic Escherichia coli strains from foxes and raccoon dogs were used as tested strains, the susceptibilities to antimicrobials were conducted on isolates of Escherichia coli.Tetracycline-resistant genes tetA, tetB, tetC, tetD, aminoglycoside-resistant genes strA-strB, aadA1, sulfisoxazole-resistant genes sul1, sul2, sul3 were detected by using conventional PCR.The results showed that the resistance rates of the 15 isolates to oxytetracycline, doxycycline, cefradine, neomycin, penicillin, sulfamonomethoxine, sulfamethazine were 100％;the resistance rates of the 15 isolates to amoxicillin, gentamicin, ceftriaxone, amikacin, spectinomycin, streptomycin, cotrimoxazole, ampicillin, ciprofloxacin were 93.33％, 53.33％, 33.33％, 73.33％, 86.66％, 86.66％, 86.66％, 66.67％, 66.67％, respectively;The detection rates of 9resistance genes tetA, tetB, tetC, tetD, strA-strB, aadA1, sul1, sul2 and sul3were 0, 46.67％, 26.67％, 13.33％, 40％, 100％, 53.33％, 46.67％and 53.33％, respectively;tetB gene had the highest detection rate in tetracycline resistance genes, aadA1 had the highest detection rate in aminoglycoside resistance gene, the detection rates of sulfonamide drug resistance genes had less difference.The result indicated that the drug resistant spectrum of 15 strains of Escherichia coli in foxes and raccoon dogs was wide and the resistance rate was high, and the prevalence of drug resistant genes was very common.关键词
狐/貉/大肠埃希菌/药敏试验/耐药基因Key words
fox/raccoon dog/Escherichia coli/drug sensitivity test/resistance gene分类
农业科技引用本文复制引用
高光平,朱利霞,王洪彬,赵希艳,高桂生,史秋梅..狐和貉源大肠埃希菌药敏试验及耐药基因检测[J].动物医学进展,2019,40(1):30-35,6.基金项目
河北省自然科学基金项目(C2015407033) （C2015407033）
河北省教育厅(SLRC2017037) （SLRC2017037）
河北省高层次人才资助项目(A2016005046) （A2016005046）
河北科技师范学院科学研究基金项目 （）
河北省科技厅(18246629G) （18246629G）
石家庄市科技局(171500953A) （171500953A）
