动物医学进展2019，Vol.40Issue(1)：36-41,6.
鸽副黏病毒灭活疫苗候选株aYQ的生物学特性研究
Biological Characteristics of Inactivated Vaccine Candidate aYQ of Pigeon Paramyxovirus
摘要
Abstract
The objective of this study was to evaluate the biological characteristics of the aYQ strain of pigeon paramyxovirus and to lay the foundation for the development of the pigeon-paramyxovirus inactivated vaccine.PPMV aYQ strain were passaged continuously in SPF chicken embryos to establish a seed batch.HA and EID50 were determined and the purity of each strain was tested;The virulence of the aYQ strain was evaluated by MDT, ICPI and IVPI.Pathogenicity of aYQ strain to chicken embryos and pigeons, genetic stability and antigen relevance were determined.PPMV aYQ strain was passaged to 10 generations in SPF chicken embryos in the absence of bacteria, mold, mycoplasma and exogenous viruses.The HA titers of various generation viruses were 1:256-1:512and the virus contents were 10-8.5-10-8.9 EID50/0.1mL.Both ICPI and IVPI of PPMV aYQ strain were 0, and the MDT was more than 120h;The 10-day-old SPF chicken embryos inoculated with 103-fold diluted PPMV aYQ strain survived at 96 hpost inoculation.Pigeons which were infected artificially at a dose of 106EID50 with PPMV aYQ strain showed no apparent clinical symptoms;The results of sequence analysis showed that there was no gene mutation in F gene of PPMV aYQ strain, and the deduced amino acid sequences were-112 GRQGRL 117-.The results of cross-hemagglutination inhibition test and cross-neutralization test showed that there was obvious antigenic difference between PPMV aYQ strain and NDV La Sota strain.The aYQ strain can be passaged in SPF chicken embryos with stable genetic and biological characteristics.It indicated that the PPMV aYQ strain possess satisfied immunogenicity, and can be used as the preferred pigeon paramyxovirus inactivated vaccine candidate strain.关键词
鸽副黏病毒/疫苗/毒种/生物学特性/遗传稳定性Key words
Pigeon paramyxovirus/vaccine/strain/biological characteristics/genetic stability分类
农业科技引用本文复制引用
赵文奇,王小蕾,刘立新,田方杰,潘洁,程水生,孙晔,蒋桃珍,刘月焕,杨志远,林健,陈光华,杨龙峰,赵际成,段会娟,丁国杰,马会籽..鸽副黏病毒灭活疫苗候选株aYQ的生物学特性研究[J].动物医学进展,2019,40(1):36-41,6.基金项目
北京市农林科学院科技创新能力建设专项项目(KJCX20161503) （KJCX20161503）
北京市农林科学院畜牧兽医研究所项目(XMS201703) （XMS201703）
