动物营养学报2019，Vol.31Issue(4)：1685-1694,10.DOI:10.3969／j.issn.1006⁃267x.2019.04.026
饲粮不同非纤维性碳水化合物/中性洗涤纤维对细毛羊氮代谢、瘤胃内环境及挥发性脂肪酸吸收的影响
Effects of Dietary Different Non?Fiber Carbohydrate／Neutral Detergent Fiber on Nitrogen Metabolism, Rumen Environment and Volatile Fatty Acid Absorption of Fine Wool Sheep
摘要
Abstract
This experiment was conducted to investigate the effects of different non﹣fiber carbohydrate/neutral detergent fiber ( NFC/NDF) on the nitrogen metabolism, rumen environment and volatile fatty acid ( VFA) absorption. Nine Inner Mongolia Ordos of sheep with permanent rumen fistula were randomly divided into three groups on the basis of body weight. The NFC/NDF in diets of groups 1, 2, 3 were 0. 35, 0. 66 and 1. 14, respectively, and the crude protein contents were all 13. 50%. The results showed as follows: 1) the NFC/NDF had no significant differences in nitrogen intake, fecal nitrogen and nitrogen deposition ( P﹥0.05) , but the urea nitrogen content in urine in group 1 was significantly higher than that in group 2 ( P＜0.05) , and sig﹣nificantly higher than that in group 3 ( P＜0.01) , and group 2 was significantly higher than group 3 ( P＜0.05) . 2) After feeding 1.5 and 3.0 h, NH3﹣N content in group 1 was significantly higher than that in group 3 ( P＜0.05) , and after feeding 4.5 h, there was no significant difference among groups ( P﹥0.05). The pH was 6.0 to 6.8, and pH in group 1 was significantly higher than that in group 3 ( P＜0.05) . 3) After feeding 1.5 h, the acetic acid content in group 1 was significantly higher than that in group 2 ( P＜0. 05) , and significantly higher than that in group 3 ( P＜0.01) . After feeding 1.5, 3.5 and 4.0 h, the propionic acid content in groups 1 and 2 was significantly lower than that in group 3 ( P＜0.05) . Before feeding and after feeding, the butyric acid content in group 1 was significantly higher than that in group 3 ( P＜0.05) . 4) The expression levels of Na+/H+exchange protein 3 ( NHE3) and down﹣regulated adenoma vector ( DRA) genes in groups 2 and 3 were significantly higher than those in group 1 ( P＜0. 05) . The expression level of monocarboxylate transporter 4 ( MCT4) gene in group 1 was significantly higher than that in groups 2 and 3 ( P＜0.05) . The expression levels of Na+/H+exchange protein 1 ( NHE1) and Na+/K+ATPase pump ( Na+/K+ATPase) in group 2 were signifi﹣cantly higher than those in groups 1 and 3 (P＜0.05). In conclusion, the rumen environment and the expres﹣sions of genes related with VFA absorption in fine wool sheep are significantly affected when dietary NFC/NDF is 0.66.[ Chinese Journal of Animal Nutrition, 2019, 31(4) :1685?1694]关键词
细毛羊/氮代谢/瘤胃内环境/VFA吸收Key words
fine wool sheep/ nitrogen metabolism/ rumen environment/ VFA absorption分类
农业科技引用本文复制引用
艳城,凌树礼,金鹿,孙海洲,桑丹,李胜利,张春华,张崇志,任晓萍,珊丹..饲粮不同非纤维性碳水化合物/中性洗涤纤维对细毛羊氮代谢、瘤胃内环境及挥发性脂肪酸吸收的影响[J].动物营养学报,2019,31(4):1685-1694,10.基金项目
国家绒毛用羊产业技术体系项目(CARS-40-12) （CARS-40-12）
内蒙古农牧业科技创新基金项目(2018CXJJM04) （2018CXJJM04）
