动物营养学报2019，Vol.31Issue(4)：1842-1849,8.DOI:10.3969／j.issn.1006⁃267x.2019.04.043
蓝藻酸解产物成分及其饲用安全性研究
Components and Feeding Safety of Cyanobacterial Acid Hydrolysate
摘要
Abstract
In order to utilize the protein resource of cyanobacteria from Taihu lake and develop new feed prod﹣uct, the amino acid composition, residual microcystin and heavy metal contents in cyanobacterial acid hydroly﹣sate were analyzed by hydrochloric acid hydrolysis method. Meanwhile, the feeding safety of cyanobacterial acid hydrolysate was also studied by using animal acute toxicity test. The results showed as follows: after hy﹣drolysis by hydrochloric acid for 14 h, a total of 17 kinds of amino acids were found in the cyanobacterial acid hydrolysate, and the total amino acid content reached to 52.63%, the livestock and poultry essential amino acid content was 30.25%, the microcystin﹣LR residual amount was 1.33 μg/kg. After hydrolysis by hydrochloric acid for 18 h, microcystin﹣LR residual amount in cyanobacterial acid hydrolysate was 0.90 μg/kg, which was lower than the standard limit of drinking water (1.0 μg/L) , and the removal ratios of microcystin﹣LR and mi﹣crocystin﹣RR were all higher than 99. 99%. The contents of heavy metals [ arsenic ( As) , plumbum ( Pb), mercury ( Hg) and chromium ( Cr) ] in the cyanobacterial acid hydrolysate was in conformity with relative na﹣tional standards after hydrolysis by hydrochloric acid for 14 h. The feeding safety of cyanobacterial acid hydrol﹣ysate was tested by mouse acute toxicity test, mouse bone marrow cell micronucleus test and mouse sperm de﹣formity test. The results showed that the half lethal dose ( LD50 ) of cyanobacterial acid hydrolysate for mice was ＞9.09 g/kg BW, which belonged to actually non﹣toxic level. The results of micronucleus test and terato﹣genic test were negative, and the cyanobacterial acid hydrolysate showed no mutagenicity and no sperm terato﹣genicity. The results indicate that the total amino acid content exceeded 50% of cyanobacterial acid hydrolysate ( cyanobacterial is hydrolyzed by hydrochloric acid for 14 h) , and it contains lysine, methionine and threonine which are easily deficient and needed to be added for livestock and poultry. The residual amounts of micro﹣cystins in products are close to the standard limit of drinking water after acid hydrolysis for more than 14 h, and the acute toxicological evaluation shows that cyanobacterial acid hydrolysate is non﹣toxic, non﹣mutagenic and azoospermic teratogenicity.[ Chinese Journal of Animal Nutrition, 2019, 31(4) :1842?1849]关键词
蓝藻/酸解产物/成分/饲用安全性/急性毒理Key words
cyanobacteria/ acid hydrolysate/ components/ feeding safety/ acute toxicity分类
农业科技引用本文复制引用
韩士群,徐玉娣,周庆,郭启金..蓝藻酸解产物成分及其饲用安全性研究[J].动物营养学报,2019,31(4):1842-1849,8.基金项目
国家水专项课题(2017ZX07203001) （2017ZX07203001）
江苏省自主创新项目[CX(18)2027] （18）
