能源环境保护2018，Vol.32Issue(6)：58-62,5.
纳米金磁免疫层析在瘦肉精残留检测中的应用研究
APPLICATION STUDY OF THE RESIDUE DETECTION OF CLENBUTEROL BY IMMUNOCHROMATOGRAPHY METHOD WITH GOLD MAGNETIC NANOPARTICLES
顾娟红 1陈军 1叶剑娜 2钱玉山2
作者信息
- 1. 苏州出入境检验检疫局检验检疫综合技术中心, 江苏 苏州 215104
- 2. 煤科集团杭州环保研究院有限公司, 浙江 杭州 311201
- 折叠
摘要
Abstract
In order to optimize the quantitative determination of low concentration clenbuterol from the food of animal origin, a immunochromatography detection system was set up using gold magnetic nanoparticles as supports. The results showed that the standard curves of clenbuterol (CLB) and rctopamine (RAC) test trips were between 0.5～50 ng/mL and 1.0～100 ng/mL, respectively. The lower limits of detection (LLOD) were 0.25 ng/mL and 0.5 ng/mL.CLB and RAC react negatively with drugs of same kind. The recovery rate is between 82.71 and 104.12 ％, which means the accuracy meets the requirements. Coefficient of variation (CV) is lower than 15 ％ with different CLB and RAC adding level, which means the the precision meets the requirements. The false positive rate and false negative rate were both 0.This method shows high sensitivity, stability and specificity for CLB and RAC detection.关键词
纳米金磁/免疫层析法/盐酸克伦特罗Key words
Coke oven flue gas/SCR/Low temperature catalyst/Solid acid/Nitrogen oxides分类
资源环境引用本文复制引用
顾娟红,陈军,叶剑娜,钱玉山..纳米金磁免疫层析在瘦肉精残留检测中的应用研究[J].能源环境保护,2018,32(6):58-62,5.
