烟草科技2019，Vol.52Issue(3)：26-32,7.
基于SSR标记的初烤烟叶品种快速鉴别
Rapid identification of cured tobacco leaf varieties based on SSR markers
摘要
Abstract
To effectively identify the varieties of cured tobacco leaves, the genomic DNA of cured leaves of 11 varieties commonly used was extracted by magnetic bead method. The fingerprint spectra based on SSR markers were constructed by PCR and agarose gel electrophoresis. The results showed that five primer pairs with high polymorphism and stability were selected from 24 SSR primer pairs. The amplified products of the five primer pairs were 100-300 bp in length and clear bands were obtained via 3% agarose gel electrophoresis. On the basis of electrophoregram of the five primer pairs, the fingerprint spectra of the 11 tobacco varieties were constructed, and the flow chart of variety identification was drawn for rapid differentiation of the 11 tobacco varieties. This method is simple, practical and convenient to identify the varieties of cured tobacco leaves.关键词
初烤烟叶/品种/SSR标记/指纹谱库Key words
Cured tobacco leaf/Variety/SSR marker/Fingerprint spectrum library分类
农业科技引用本文复制引用
孙九喆,杨金初,苏东嬴,王二彬,王君婷,张顺峰,李萌,马林..基于SSR标记的初烤烟叶品种快速鉴别[J].烟草科技,2019,52(3):26-32,7.基金项目
云南中烟工业有限责任公司科技项目"高压均质技术在卷烟香料生产中的应用研究"(2017GY07). （2017GY07）
