烟草科技2019，Vol.52Issue(4)：1-8,8.
高、低硝态氮营养条件下烟草根系基因表达谱及代谢途径的差异分析
Analysis of gene expression profile and metabolic pathway of tobacco root at high and low levels of nitrate nitrogen
摘要
Abstract
To investigate the responses of tobacco roots to nitrate signals, taking flue-cured tobacco variety K326 as experimental material, seedling culture and nitrogen stress experiments were conducted in a greenhouse with controlled light, temperature and humidty (14 h light, 10 h dark, temperature 28 ℃, relative humidity 65%-75%) and transcriptome sequencing technique was used to study the differentially expressed genes (DEGs) in tobacco roots at high (NO3-5.0 mmol/L) and low (NO3-0.1 mmol/L) levels of nitrate nitrogen applying after 0, 6, 12 and 24 h. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed that the expressions of some DEGs in sub-cluster increased first and then decreased at high and low levels of nitrate nitrogen applying after 6 h. These genes were mapped in phenylpropanoid biosynthesis and the metabolic pathways of alanine, aspartic acid and glutamic acid. The results indicated that the metabolism pathways of amino acids and carbon/nitrogen were important to respond nitrogen signal. However, some genes were involved in the biosynthesis of tropone, piperidine and pyridine alkaloid. The metabolism pathways of pyrimidine, purine, fructose, mannose, starch and sucrose down-regulated at the low level of nitrate nitrogen while they up-regulated at the high level of nitrate nitrogen, which suggested that the alkaloid synthesis and energy metabolism in flue-cured tobacco roots were inhibited at the low level of nitrate nitrogen and were promoted at the high level of nitrate nitrogen.关键词
烟草/氮营养/硝酸根信号/转录组分析Key words
Tobacco/Nitrogen nutrition/Nitrate signal/Transcriptome analysis分类
轻工纺织引用本文复制引用
张玉宁,史宏志,王景,周炎,杨惠娟..高、低硝态氮营养条件下烟草根系基因表达谱及代谢途径的差异分析[J].烟草科技,2019,52(4):1-8,8.基金项目
河南中烟工业有限责任公司项目"'黄金叶'品牌基地单元浓香烤烟品种配套技术研究与应用"(HN102013008). （HN102013008）
