中国烟草科学2019，Vol.40Issue(2)：8-15,8.DOI:10.13496/j.issn.1007-5119.2019.02.002
不同产区晾晒烟资源多样性的鉴定与评价
Identification and Evaluation of the Diversity of Air/Sun-cured Tobacco Germplasm Resources in Different Producing Areas
摘要
Abstract
In order to improve the utilization efficiency and to develop new utilization approaches of air/sun-cured tobacco germplasm resources, we conducted agronomic trait characterization, chemical composition determination and genetic diversity analyses using 93 air/sun-cured tobacco germplasms grown in five tobacco cultivation areas in China. The results showed that the agronomic traits and chemical components of different germplasms varied greatly. The coefficient of variation ranged from 7.60% to 70.17% in different traits. The coefficient of variation of the growth period is the smallest, and that of the total chlorine is the largest. The nicotine content ranged from 1.20% to 8.63%, and potassium content ranged from 0.91% to 4.18%. In addition, 43 pairs of primers with specific amplification were screened from 580 pairs of SSR primers, and 146 polymorphic bands were obtained, of which the polymorphic ratio was 97.99%. The Shannon's index was 0.9379, Nei's diversity index was 0.5356. The 93 tobacco germplasms were clustered into 3 groups with the genetic similarity coefficient of 0.50 by cluster analysis. Germplasms from different cultivation areas in many cases were not clustered together. The genetic consistency between the population in the southwest cultivation area and the Huanghuai cultivation area was 0.9580, while the genetic consistency between the southwest cultivation area and the northeast cultivation area was 0.7820. These results will provide reference for screening and matching the parental germplasms for breeding of excellent mixed cigarette and oral tobacco.关键词
晾晒烟/农艺性状/化学成分/SSR分子标记Key words
air/sun-cured tobacco/agronomic traits/chemical composition/SSR molecular marker分类
农业科技引用本文复制引用
鞠馥竹,刘艳华,赵文涛,刘元德,窦玉青,杨菁,刘莉,张忠锋,张洪博,闫宁..不同产区晾晒烟资源多样性的鉴定与评价[J].中国烟草科学,2019,40(2):8-15,8.基金项目
中国烟草总公司科技重大专项项目"口含烟烟叶原料质量评价研究"[10201501004(XX-04)] （XX-04）
中国农业科学院科技创新工程(ASTIP-TRIC05) （ASTIP-TRIC05）
