中国烟草科学2019，Vol.40Issue(2)：44-51,8.DOI:10.13496/j.issn.1007-5119.2019.02.007
烟草PP2C家族基因降雨量响应表达模式分析
Expression Analysis of PP2C Family Members in Response to Rainfall in Tobacco
摘要
Abstract
PP2C is a class of monomeric serine/threonine protein phosphatases that are widely involved in multiple signal transduction pathways in organisms. Identification and functional studies of the PP2C gene family have been reported in a variety of crops. In order to ascertain the expression characteristics of tobacco PP2C gene family members under different rainfall conditions, the expression of PP2C family genes in tobacco leaves at 40 and 60 days after transplanting was analyzed by RNA-Seq technique through simulated artificial quantitative irrigation in plastic greenhouse and field experiments with different rainfall. The results showed that the expression of 18 tobacco PP2C family genes was significantly affected by rainfall and could be divided into 9 subfamilies. The expression of six PP2C family members decreased with increasing rainfall, and the expression of four PP2C family members increased with increasing rainfall. In this study we have preliminarily identified the types and expression characteristics of the PP2C gene family affected by rainfall in Yunyan 87, providing reference for drought-resistant breeding and drought-resistant cultivation.关键词
烟草/降雨量/PP2C/生物信息学Key words
tobacco/rainfall/PP2C/bioinformatics分类
农业科技引用本文复制引用
何晓健,张柳,王戈,程亚东,毛自朝,杨焕文..烟草PP2C家族基因降雨量响应表达模式分析[J].中国烟草科学,2019,40(2):44-51,8.基金项目
中国烟草总公司四川省公司资助项目"四川优质雪茄烟叶调制设施及配套调制技术研究"(SCYC201809) （SCYC201809）
浙江中烟工业有限责任公司资助项目"浙江中烟-河南农大基地技术依托服务"(2017330000341540) （2017330000341540）
