中国油料作物学报2018，Vol.40Issue(6)：745-754,10.DOI:10.7505/j.issn.1007-9084.2018.06.002
甘蓝型油菜BnDMC1. A01基因的分离及序列分析
Isolation and sequence analysis of meiotic gene BnDMC1.A01 in Brassica napus
摘要
Abstract
Synthetic B. napus is an important type of materials for genomic research and germplasm improvement. But there are abnormalities in the process of meiosis which lead to genetic instability and pollen fertility decline in synthesized B. napus. For better understanding molecular mechanism of meiosis, sequence of DMC1 homologous, the important plants meiotic gene was isolated from B. napus. Primers were designed based on conserved cDNA sequences from homologous genes, and the DMC1 sequence on A01 chromosome was isolated from B. napus.The amino acid sequence alignment showed that BnDMC1. A01 encoded protein containing typical conserved domains of DMC1 had also been reported in rice and maize. At the same time, 6 haplotypes of the gene were obtained by sequencing analysis of BnDMC1. A01 in 19 accessions of B. napus; 2 SNPs resulted in mutation of amino acid residues were found and they distributed in all 3 types of B. napus. Gene expression showed that BnDMC1. A01 expression level in synthesized B. napus was higher than that in traditional B. napus. However, whether these variations in amino acid or gene expression cause abnormal meiosis in synthesized B. napus still need further elucidation.关键词
甘蓝型油菜/减数分裂/DMC1/基因分离/单倍型Key words
Brassica napus/meiotic/DMC1/gene isolation/haplotype分类
农业科技引用本文复制引用
鲁丹丹,李保全,安素妨,徐文,侯锦娜..甘蓝型油菜BnDMC1. A01基因的分离及序列分析[J].中国油料作物学报,2018,40(6):745-754,10.基金项目
国家自然科学基金(31501341) （31501341）
河南省农业科学院科研发展专项(YNK20177516) （YNK20177516）
河南省基础与前沿技术研究计划项目(162300410153) （162300410153）
