国际医药卫生导报2019，Vol.25Issue(7)：1010-1014,5.DOI:10.3760/cma.j.issn.1007-1245.2019.07.001
丹参对高血压肥大心肌细胞中黏着斑激酶表达的影响
Influence of salvia miltiorrhiza on expression of focal adhesion kinase in cardiac myocytes of hypertrophic ventricle
摘要
Abstract
Objective To analyze the influence of salvia miltiorrhiza on the expression of focal adhesion kinase (FAK) in cardiac myocytes of hypertrophic ventricle,and to explore the mechanism of salvia niltiorrhiza in the inhibition of cardiac hypertrophy.Methods Spontaneously hypertensive rats (SHHR) were used as research objects.The expression and distribution of FAK in left ventricular myocardial cells were detected by immunofluorescent labeling,confocal microscopy,and Western blotting,and were compared among the salvia miltiorrhiza intervention group (SHHR-B),the captopril intervention group (SHHR-C),and the control rats (SHHR-A).Results Compared with the WKY rats,the expression of FAK was significantly increased in total protein,cytomembrane protein,and cyteblast protein (all P ＜ 0.01),but not in cytoplasm protein (P ＞ 0.05).Compared with that in the SHHR-A group,the expression of FAK in SHHR-B rats was decreased in total protein,cytomembrane protein,and cyteblast protein,P ＜ 0.01),but that in cytoplasm protein remained unchanged (P ＞ 0.05) in the SHHR-B group.Confocal microscopy showed that FAK accumulated significantly in the cytomembrane of the SHHR rats,and salvia miltiorrhiza could reduce the distribution of FAK in cytomembrane and cyteblast.Conclusions Salvia miltiorrhiza can inhibit the expression of FAK in total protein,cytomembrane protein,and cyteblast protein of cardiomyocytes,reduce the accumulation of FAK in cell membrane and cyteblast,and inhibit myocardial hypertrophy.关键词
黏着斑激酶/高血压/心肌肥大/丹参Key words
Focal adhesion kinase/Hypertension/Myocardial hypertrophy/Salvia miltiorrhiza引用本文复制引用
林常青,吕小飞,林俊红,赖锦斌,徐力堃,邹燕敦..丹参对高血压肥大心肌细胞中黏着斑激酶表达的影响[J].国际医药卫生导报,2019,25(7):1010-1014,5.基金项目
广东省中医药局项目(20151036)Project of Guangdong Bureau of Traditional Chinese Medicine (20151036) （20151036）
