山西农业大学学报（自然科学版）2019，Vol.39Issue(2)：14-20,7.DOI:10.13842/j.cnki.issn1671-8151.201808002
谷子种子愈伤组织的诱导与植株再生
Effects of different kinds of hormones on the callus induction and plant regeneration of millet seeds
摘要
Abstract
[Objective]Effects of varioustypes of hormones at different concentrations on callus induction and plant regeneration of Jingu 21 seedlings were investigated.Present study was aimed to determine the optimal hormone ratio for better callus in-duction and differentiation,and to establish a stable millet regeneration system with high efficiency.[Methods]The matured seeds of Jingu 21 millet were employed as explant material,and effects of different types of hormones at different concentra-tions on callus induction and plant regeneration of Jingu 21 were studied.[Results]By adding 2,4-D to MS medium,the callus induction rate was enhanced and better than that of NAA,and the quality of callus was improved by adding a certain concen-tration of 6-BA to the medium.When the concentration of 4-D and 6-BA were 2.0 mg·L-1 and 0.2 mg·L-1 ,respectively,the callus induction rate reached the highest peak at 92.86%.In the process of callus differentiation,the effect of adding 6-BA was significantly better than that of ZT,with the highest callus differentiation rate (1 3.33%)occurred at the concentration of 6-BA at 1-0 mg·L-1 .In addition,transferring millet seedlings to MS medium induced root development.[Conclusion]The opti-mal medium for inducing callus was MS mixed with 2.0 mg·L-1 2,4-D and 0.2 mg·L-1 6-BA,and the best differentiation medium combination was MS plus 1.0 mg·L-1 6-BA.关键词
谷子种子/愈伤组织/诱导/植株再生Key words
Millet seeds/Callus/Induction/Plant regeneration分类
农业科技引用本文复制引用
王晓璐,尹艺臻,温银元,原向阳,王育选,赵娟..谷子种子愈伤组织的诱导与植株再生[J].山西农业大学学报（自然科学版）,2019,39(2):14-20,7.基金项目
国家现代农业产业体系(CARS-06-13.5-A28) （CARS-06-13.5-A28）
山西农业大学青年拔尖创新人才支持计划(TYIT201406) （TYIT201406）
谷子全程机械化产业技术开发(2015-TN-09) （2015-TN-09）
山西省科技重点研发项目(2015-TN-09-05) （2015-TN-09-05）
国家自然科学基金(31301269) （31301269）
