山西农业大学学报（自然科学版）2019，Vol.39Issue(2)：27-32,6.DOI:10.13842/j.cnki.issn1671-8151.201809060
转乙醇酸脱氢酶(GD H )基因棉花的获得及表型鉴定
Phenotypic identification of genetically modified cotton with glycolate dehydrogenase (GDH)gene
摘要
Abstract
[Obj ective]Reducing photorespiration rate can effectively improve photosynthetic efficiency andincrease the plant biomass.Present study was designed to introduce bacterial glycolic acid pathway into plants and to establish a photorespiration bypass which directly release CO2 into chloroplasts and promote Rubisco carboxylation resulting in photosynthetic efficiency improvement.[Methods]In the study,glycolate dehydrogenase gene (GD H )from E.coliwas transferred into upland cotton variety R1 5 by Agrobacterium tumefaciens mediated method,and regenerated plants were obtained by somatic callus induction in antibiotic-containing screening medium.[Results]Molecular detection re-vealed that GD H was successfully introduced into cotton receptor and a total of 3 6 transgenic plants were obtained. The photosynthetic parameterswere measured and the phenotypes of plants in the field were determined.Results indi-cated that the net photosynthetic rate of several transgenic plants was enhancedby 26.99%~37.29%,and plant height wasincreased by 10.07%~16.18%.Fresh leaf weight and dry weightwere increased 21.59%~44.25% and 1 1.06%~17.79%,respectively,compared to that of control plants.These results demonstrated that photorespiration rate of GD H gene modified plants was reduced and plant biomass were significantly improved.[Conclusion]This study pro-vided a new way to screen cotton germplasm resources with high photosynthetic efficiency and yield.关键词
光呼吸/乙醇酸脱氢酶/转基因/生物量Key words
Photorespiration/Glycolate dehydrogenase/Transgene/Biomass分类
农业科技引用本文复制引用
张树伟,张义茹,马燕斌,李换丽,Rupert Fray,韩渊怀..转乙醇酸脱氢酶(GD H )基因棉花的获得及表型鉴定[J].山西农业大学学报（自然科学版）,2019,39(2):27-32,6.基金项目
181 国家基金培育匹配项目(2017GPY05) （2017GPY05）
山西功能农业共性关键技术研究与示范(201703D211001) （201703D211001）
