南京医科大学学报（自然科学版）2019，Vol.39Issue(4)：500-504,5.DOI:10.7655/NYDXBNS20190407
远端缺血后适应通过抑制RAGE的表达实现对心肌的保护作用
Remote ischemic postconditioning protects myocardium by inhibiting the expression of RAGE
摘要
Abstract
Objective:This study aimed to investigate the role of receptor for advanced glycation end products (RAGE) in improving myocardial ischemia and reperfusion after remote ischemic postconditioning. Methods:Thirty-four C57/B6 mice aged 8-9 weeks were randomly divided into six groups:the sham operation group (sham), the FPS-ZM1 control group (FZM1), the ischemia and resperfusion group (IR), the FPS-ZM1 intervention group (FZM1+IR), the RIPostC group (RIPostC+IR) and the RIPostC intervention group (RIPostC+FZM1+IR). The anterior descending coronary artery was ligated to create myocardial ischemia-reperfusion model of mouse.The left ventricular ejection fraction (LVEF) and left ventricular shortening fraction (LVFS) of mice were detected by mouse cardiac ultrasonography, and the inflammatory factor IL-6, NF-κB P65 protein and RAGE protein were detected by enzyme-linked immunoassay (ELISA) and Western blotting. Results:①RIPostC significantly increased LVEF (P ＜ 0.01) and LVFS (P ＜ 0.01) compared with the I/R group. ②Compared with the IR group, RIPostC significantly reduced the expression of RAGE (P ＜ 0.001). ③RIPostC significantly reduced the expression of NF-κB P65 (P ＜ 0.001) and IL-6 (P ＜ 0.001) compared with the I/R group.Conclusion:RIPostC was effective in protecting against myocardial ischemia-reperfusion injury. The cardioprotective effects of RIPostC may be achieved directly by inhibiting the expression of RAGE and thereby reducing the inflammatory response.关键词
缺血再灌注/晚期糖基化终末产物受体/远端缺血后适应Key words
ischemic reperfusion/advanced glycation end-product receptor/remote ischemic postconditioning分类
医药卫生引用本文复制引用
冯楚炎,王向明,屠甜甜,Deeraj Mungun,许婷婷,徐婉莹,郭妍..远端缺血后适应通过抑制RAGE的表达实现对心肌的保护作用[J].南京医科大学学报（自然科学版）,2019,39(4):500-504,5.基金项目
江苏省干部保健科研课题(BJ18017) （BJ18017）
