南京医科大学学报（自然科学版）2019，Vol.39Issue(4)：505-512,8.DOI:10.7655/NYDXBNS20190408
HDGF过表达促进胶质瘤细胞体外迁移与侵袭及β-catenin磷酸化
HDGF overexpression enhances migration and invasion of glioma cells by promoting β-catenin phosphorylation in vitro
摘要
Abstract
Objective:To investigate the effects of up-regulation of hepatoma-derived growth factor (HDGF) expression on the biological behavior of glioma cells in vitro. Methods: The effects of overexpression of HDGF on the proliferation of malignant glioma cell line DBTRG were examined by MTS and bromodeoxyuridine (BrdU) incorporation experiments. The effects of HDGF up-regulation on the migration and invasion of DBTRG in vitro were investigated by Wound-healing assay, Transwell assay and Matrigel invasion assay, respectively. Western blot and immunofluorescence were used to determine the expression and subcellular localization of cell adhesion molecules. Results: There was no significant difference in the proliferation rate and the proportion of BrdU positive cells between the overexpressing group and the control group. Compared with the control group, the numbers of migration and invasion cells in the HDGF overexpression group were significantly increased. In addition, overexpression of HDGF increased N-cadherin expression but decreased β-catenin protein levels. Furthermore, HDGF did not affect the nuclear translocation of β-catenin, but promoted its phosphorylation. Conclusion: HDGF enhances migration and invasion of DBTRG glioma cells in vitro by promoting β-catenin phosphorylation.关键词
HDGF/胶质瘤/迁移/侵袭/β-cateninKey words
HDGF/glioma/migration/invasion/β-catenin分类
医药卫生引用本文复制引用
傅松,龙卫国,张爱霞..HDGF过表达促进胶质瘤细胞体外迁移与侵袭及β-catenin磷酸化[J].南京医科大学学报（自然科学版）,2019,39(4):505-512,8.基金项目
江苏省自然科学基金(BK2011767) （BK2011767）
