南京医科大学学报（自然科学版）2019，Vol.39Issue(4)：528-533,562,7.DOI:10.7655/NYDXBNS20190411
PGAM1在涎腺腺样囊性癌中的表达及其临床和生物学意义
Expression of PGAM1 in salivary adenoid cystic carcinoma and its clinical and biological significances
摘要
Abstract
Objective:To study the expression of phosphoglycerate mutase1 (PGAM1) in salivary adenoid cystic carcinoma (SACC and its clinical and biological significance. Methods:PGAM1 expression was detected by immunohistochemistry in 31 cases of SACC and 25 normal glands, and the correlation with the clinical pathology parameter was analyzed. The highly metastatic human salivary adenoid cystic carcinoma cell line (SACC-LM) was transient transfected by PGAM1 siRNA interference clips. Cell cycle changes, cell proliferation, migration, invasion were evaluated. The changes of matrix metalloproteinase (MMP)-2, MMP-9, N-cadherin, E-cadherin and vimentin expression were examined before and after the interference by Western blot. Results:PGAM1 was expressed at a low level in 25 normal glands. In 31 cases of SACC, 20 cases (64.5％) expressed PGAM1 at a high level, while 11 cases (35.5％) expressed at a low level. The PGAM1 expression was significantly higher in the solid pattern than that in the tubular and cribriform pattern (P＜0.05). The expression of PGAM1 had no correlation with gender, age or tumor location (P＞0.05). The down-regulation of PGAM1 had no effect on the proliferation capability and cell cycle of SACC-LM cells (P＞0.05), while cells migration and cell invasion were inhibited significantly after PGAM1 was down-regulated (P＜0.05). The expression of MMP-2 and MMP-9 was also down-regulated after PGAM1 was inhibited (P＜0.05). Conclusion:The expression of PGAM1 was associated with pathological types of SACC;down-regulation of PGAM1 can significantly inhibit the migration and invasion of adenoid cystic carcinoma.关键词
PGAM1/涎腺腺样囊性癌/病理/迁移/侵袭Key words
PGAM1/salivary adenoid cystic carcinoma/pathology/migration/invasion分类
医药卫生引用本文复制引用
罗亚东,武和明,杜洪明,宋海洋,马虞楠,李萌,丁旭..PGAM1在涎腺腺样囊性癌中的表达及其临床和生物学意义[J].南京医科大学学报（自然科学版）,2019,39(4):528-533,562,7.基金项目
国家自然科学基金(81600908) （81600908）
江苏省医学创新团队(CXTDA2017036) （CXTDA2017036）
