摘要
Abstract
Objective To investigate the effect of emodin(EMO) pretreatment on pyroptosis induced by LPS /ATP in human umbilical vein endothelial cells(HUVECs). Methods HUVECs with good growth status were randomly divided into three groups: normal control group(C group),LPS /ATP group,LSP/ATP + EMO group(EMO group). The HUVEC cells in LPS /ATP group were stimulated with 1 μg /ml lipopolysaccharide(LPS) for 24 h,and then stimulated with 5. 5 mmol /L adenosine triphosphate(ATP) for 4 h. The HUVEC cells in EMO group were pretreated with emodin 60 μmol /L for 24 h,the cells were stimulated with 1 μg /ml lipopolysaccharide(LPS) for 24 h,and then stimulated with 5. 5 mmol /L adenosine triphosphate(ATP) for 4 h. The levels of LDH and ROS in the supernatant were detected by ELISA and the changes of NLRP3,caspase-1,IL-1β,IL-18 protein were detected by Western blot. Results Compared with normal control group,the levels of LDH and ROS in LPS /ATP group were increased significantly(P ＜ 0. 05), and the expression of NLRP3,caspase-1,IL-1β and IL-18 was increased significantly(P ＜ 0. 05). Compared with LPS /ATP group, the levels of LDH and ROS in the supernatant of the cells was decreased significantly in EMO group(P ＜ 0. 05),and the expression of NLRP3,caspase-1,IL-1β and IL-18 in the cells was decreased significantly(P ＜ 0. 05). Conclusion Emodin can inhibit the cell apoptosis and reduce the cell injury,which may be related to the inhibition of intracellular ROS by emodin.关键词
大黄素/细胞焦亡/人脐静脉内皮细胞/脂多糖/三磷酸腺苷Key words
emodin/pyroptosis/human umbilical vein endothelial cells/LPS/ATP分类
医药卫生
