山西医科大学学报2019，Vol.50Issue(4)：421-426,6.DOI:10.13753/j.issn.1007-6611.2019.04.008
乳腺癌干细胞标志物CD44/CD24与ALDH1的相关性分析
Correlation between breast cancer stem cell marker CD44/CD24 and ALDH1
摘要
Abstract
Objective To analyze the positive ratio of breast cancer stem cells marker CD44/CD24 and acetaldehyde dehydrogenase 1 (ALDH1) and their correlation. Methods Four cases of breast cancer metastatic pleural effusion and eight breast cancer cell lines were collected and cultured, and then stained with two breast cancer stem cell markers (CD44/CD24 and ALDH1). The ratios of CD44+CD24-and ALDH1+subpopulation were detected by flow cytometry, and the correlation between two markers was analyzed.Results The ratios of CD44+CD24-subpopulation in 2 of 8 breast cancer cell lines were higher than 85％, while the ratios in the rest cell lines were below 5％. The ratio of ALDH1+subpopulation was relatively low, from 0. 98％ to 25. 2％. In 4 cases of pleural effusion, the proportion of CD44+CD24-subpopulation was high in total, however, the ratio of ALDH1+subpopulation fluctuated greatly.And there was no significant correlation between the expression of CD44/CD24 and ALDH1 (r =-0. 168 3, P = 0. 383). Furthermore, the ratio of CD44+CD24-subpopulation in ALDH1+, ALDH1-and unlabeled cells showed no difference. Conclusion The expression of CD44/CD24 and ALDH1 in breast cancer cell is independent and there is no correlation between them.关键词
乳腺癌干细胞/ALDH1/CD44/CD24/相关性Key words
breast cancer stem cells/ALDH1/CD44/CD24/correlation分类
医药卫生引用本文复制引用
樊新宇,梦遥,盛长斌,何朵,李玲..乳腺癌干细胞标志物CD44/CD24与ALDH1的相关性分析[J].山西医科大学学报,2019,50(4):421-426,6.基金项目
国家自然科学基金面上项目(81872349) （81872349）
