山西医科大学学报2019，Vol.50Issue(4)：427-432,6.DOI:10.13753/j.issn.1007-6611.2019.04.009
槐糖脂通过线粒体途径诱导人胃癌SGC-7901细胞凋亡的机制
Mechanism of apoptosis of human gastric cancer cell line SGC-7901 induced by sophorolipid through mitochondrial pathway
摘要
Abstract
Objective To investigate the inhibitory effect of sophorolipid on the proliferation of human gastric cancer cell line SGC-7901 and to elucidate the mechanism of mitochondrial apoptosis of SGC-7901 induced by sophorolipid. Methods Human gastric cancer cell line SGC-7901 was treated with different concentrations of sophorolipid (40, 80, 160, 320 μg/ml), and human gastric cancer cell line SGC-7901 without sophorolipid treatmen was chosen as blank control group. Changes of SGC-7901 in each sophorolipid group were observed. Changes of cell activity was observed under inverted microscope after sophorolipid treatment, and the survival rate of SGC-7901 was detected by CCK-8 method. DAPI staining was used to observe the changes of cell morphology after sophorolipid treatment under fluorescence microscope. Rhodamine 123 staining was used to detect mitochondrial membrane potential levels in each group by flow cytometry. The expression levels of Bcl-2, Bax, Cyt-C, Caspase 3 and Caspase 9 in each group were detected by Western blot method. Results Compared with blank control group, the activity of human gastric cancer cell line SGC-7901 decreased in sophorolipid groups, the survival rate of SGC-7901 decreased significantly (P ＜ 0. 05), and the decrease was positively correlated with the dose of sophorolipid, and the morphology showed obvious apoptosis-like changes. Compared with blank control group, the mitochondrial membrane potential levels of SGC-7901 decreased significantly in sophorolipid groups (P ＜ 0. 05), the expression of Bax protein increased significantly (P ＜ 0. 05), the expression of Bcl-2 protein decreased significantly (P ＜ 0. 05), and the levels of Cyt-C, Caspase 3 and Caspase 9 increased significantly (P ＜ 0. 05). Conclusion Sophorolipid can inhibit the proliferation of human gastric cancer cell line SGC-7901, and the mechanism may be related to the apoptosis of SGC-7901 induced through mitochondrial apoptosis pathway.关键词
胃癌/槐糖脂/线粒体途径/细胞凋亡Key words
gastric cancer/sophorolipid/mitochondrial pathway/apoptosis分类
医药卫生引用本文复制引用
成利娟,杨莉,蒋磊,侯梅,马晓静..槐糖脂通过线粒体途径诱导人胃癌SGC-7901细胞凋亡的机制[J].山西医科大学学报,2019,50(4):427-432,6.基金项目
国家自然科学基金青年科学基金项目(31400049) （31400049）
