山西医科大学学报2019，Vol.50Issue(4)：468-472,5.DOI:10.13753/j.issn.1007-6611.2019.04.017
香烟烟雾提取物对人颌骨来源成骨细胞矿化能力的影响
Effects of cigarette smoke extracts on mineralization ability of human jawbone-derived osteoblasts
张志宏 1李钧 2王丁 1庄润涛3
作者信息
- 1. 北京民航总医院口腔科,北京 100123
- 2. 首都医科大学附属北京口腔医院
- 3. 北京交通大学社区卫生服务中心
- 折叠
摘要
Abstract
Objective To observe the effects of cigarette smoke extract on the mineralization ability of human jawbone-derived osteoblasts in vitro. Methods Osteoblasts were obtained from 30 healthy subjects with dental implants in the Department of Stomatology, Beijing Stomatological Hospital and the General Hospital of Civil Aviation, Affiliated to Capital Medical University, and then and isolated. The final concentration of extract was 0. 5％ (low concentration group) and 3％ (high concentration CSE group), and smoke-free extract was used as negative control group. The proliferation ability was detected by MTT assay, the in vitro mineralization ability of human jawbone-derived osteoblasts was detected by alkaline phosphatase staining method, and the expression of ALP, BSP and COL-1 in each group was detected by RT-PCR. Results There was no significant difference in the proliferation and mineralization of jawbonederived osteoblasts between control group and low-concentration CSE group (P＞ 0. 05), and there was also no significant difference in the expression of ALP, COL-1 and BSP (P＞ 0. 05). The proliferation and mineralization ability of osteoblasts in high concentration group were lower than those in control group and low concentration CSE group (P ＜ 0. 05), and the expression of ALP, COL-1 and BSP was also lower than that of control group and low concentration group (P ＜ 0. 05). Conclusion CSE can affect the formation of osteoblast mineral scaffolds and the deposition of minerals, reduce the quantity and quality of new bone formation, and reduce the degree of calcification of extracellular matrix of osteoblasts, thus affecting the osseointegration of implants.关键词
成骨细胞/烟雾提取物/增殖/矿化能力/种植体Key words
osteoblast/cigarette smoke extract/proliferation/mineralization ability/implant分类
医药卫生引用本文复制引用
张志宏,李钧,王丁,庄润涛..香烟烟雾提取物对人颌骨来源成骨细胞矿化能力的影响[J].山西医科大学学报,2019,50(4):468-472,5.
