中国医科大学学报2019，Vol.48Issue(3)：210-215,6.DOI:10.12007/j.issn.0258-4646.2019.03.004
Notch信号通路促进滑膜肉瘤细胞SW982增殖和侵袭的研究
Notch Signaling Promotes Proliferation and Migration of SW982 Synovial Sarcoma Cells
摘要
Abstract
Objective To investigate the effect of the Notch signaling pathway on the proliferation and invasion of human SW982 synovial sarcoma cells. Methods SW982 cells and normal human synovial cells were routinely cultured, and the expression of proteins related to the Notch pathway was compared. The Notch signaling pathway was manipulated by NICD1 overexpression, CFB1 shRNA lentivirus, and the γ-secretase inhibitor, DAPT. CCK-8 and wound healing assays were carried out to investigate the role of the Notch signaling pathway in SW982 cells. Results The Notch signaling pathway clearly showed higher activity in human SW982 synovial sarcoma cells than in normal human synovial cells (P ＜ 0.05). The proliferation and invasion of SW982 cells were significantly upregulated by overexpressing NICD1; however, were suppressed by downregulating the Notch signaling pathway using CFB1 shRNA or DAPT (P ＜ 0.05). Conclusion Our findings demonstrate that the proliferation and invasion of human SW982 synovial sarcoma cells are dependent on Notch signaling pathway activity.关键词
滑膜肉瘤/Notch信号通路/增殖/侵袭Key words
synovial sarcoma/Notch signalling pathway/proliferation/invasion分类
医药卫生引用本文复制引用
高天,余铃,李舒,刘佳勇,白楚杰,薛瑞峰,张路,方志伟,樊征夫..Notch信号通路促进滑膜肉瘤细胞SW982增殖和侵袭的研究[J].中国医科大学学报,2019,48(3):210-215,6.基金项目
国家自然科学基金(81802689) （81802689）
