中国医科大学学报2019，Vol.48Issue(3)：236-239,4.DOI:10.12007/j.issn.0258-4646.2019.03.009
miR-362-3p在喉癌组织中的表达及其对喉癌Hep-2细胞迁移的影响
miR-362-3p Expression in Laryngeal Cancer Tissues and Its Effect on Migration of Hep-2 Cells
摘要
Abstract
Objective To explore miR-362-3 p expression in laryngeal cancer tissues and its effect on migration of Hep2 cells. Methods miR-362-3 p expression in 50 pairs of tumor and adjacent normal tissues was detected by real-time PCR after sample collection. The relationships between miR-362-3 p expression and clinical pathological characteristics in patients with laryngeal cancer were analyzed.mi R-362-3 p mimic, inhibitor, and control microRNA were transfected into Hep-2 cells. Transfection efficiency was determined by real-time PCR. Wound-healing and transwell assays were used to evaluate Hep-2 migration. Results miR-362-3 p expression was significantly higher in cancer tissues than in adjacent normal tissues (P ＜ 0.05). miR-362-3 p expression was statistically significantly related to node metastasis and clinical stage. Transfection with miR-362-3 p mimic and inhibitor significantly increased and decreased Hep-2 cell migration, respectively (both P ＜ 0.05). Conclusion miR-362-3 p is up-regulated in laryngeal cancer tissues and promotes laryngeal cancer cell migration, suggesting that it acts as a potential oncogene in laryngeal cancer.关键词
miR-362-3p表达/喉癌/迁移Key words
miR-362-3p expression/laryngeal cancer/migration分类
医药卫生引用本文复制引用
赵越,孙媛媛,佟雪,岳鹏杰,富伟能..miR-362-3p在喉癌组织中的表达及其对喉癌Hep-2细胞迁移的影响[J].中国医科大学学报,2019,48(3):236-239,4.基金项目
辽宁省重点研发计划(2017225021) （2017225021）
辽宁省高等学校基本科研项目(LQNK201726) （LQNK201726）
