安徽农业大学学报2018，Vol.45Issue(6)：996-1003,8.DOI:10.13610/j.cnki.1672-352x.20190102.005
蜗牛酶辅助提取白芽奇兰茶多糖及其抗氧化活性
Polysaccharides extraction from Baiyaqilan tea by snail enzyme and it antioxidant activity
摘要
Abstract
A snail enzyme-assisted extraction of polysaccharides from Baiyaqilan tea was used to improve the utilization ratio of Baiyaqilan tea. Based on the single-factor experiment, a four-factor and three-level experiment was designed by the Box-Behnken method using extraction yield of polysaccharides as response value, enzymolysis temperature, enzyme dosage, enzymolysis time and pH as factors. The enzymatic extraction technology of polysaccharide from Baiyaqilan tea was optimized using response surface methodology by Design-Expert 8.05 b software. The scavenging capacity of DPPH, OH and O2- free radicals was used to evaluate its antioxidant activity. The results showed that the optimum extraction parameters were as follows: temperature 51℃, enzyme dosage 1.2％, enzymolysis time 55 min and pH 5.6, and the extraction yield was up to 93.81 mg·g-1. The extraction rate of this process increased 77.74 ％ than that of the hot water extraction without snail enzyme. Experiment results showed that there was a significant dose-effect relationship between the scavenging effects of polysaccharides from DPPH, OH and O2- free radicals, the IC50 values of DPPH, OH and O2- free radicals were 80.65, 204.39 and 428.10 mg·L-1, respectively.关键词
白芽奇兰/茶多糖/蜗牛酶/抗氧化Key words
Baiyaqilan tea/tea polysaccharides/snail enzyme/antioxidant activity分类
轻工纺织引用本文复制引用
陈文娟,陈建福,卢惠婷,庄远红..蜗牛酶辅助提取白芽奇兰茶多糖及其抗氧化活性[J].安徽农业大学学报,2018,45(6):996-1003,8.基金项目
漳州市自然科学基金(ZZ2017J43) （ZZ2017J43）
福建省高校杰出青年科研人才培育计划(闽教科[2015]54号 （）
福建省中青年教师教育科研项目(JAT171090)共同资助 （JAT171090）
