安徽农业大学学报2018，Vol.45Issue(6)：1107-1111,5.DOI:10.13610/j.cnki.1672-352x.20190102.001
天冬氨酸修饰的纳米硒 (PEG2000-ASP@Se) 对间充质干细胞增殖与分化能力的影响
Osteoblastic differentiation of mesenchymal stem cell on the aspartic acid functional selenium nanoparticles
摘要
Abstract
In this study, aspartic acid (ASP) and PEG2000 functional selenium nanoparticles (PEG2000-ASP@Se) were used for stem cell research to identify their effects on mesenchymal stem cells (hMSCs) multiplication and differentiation. The morphology and surface state of PEG2000-ASP@Se were characterized by transmission electron microscope (TEM). The spherical PEG2000-ASP@Se presented monodisperse and homogeneous spherical structure with an average diameter of (154±2) nm. Before determining the role of PEG2000-ASP@Se in hMSCs osteogenic and differentiation, its cytotoxicity was assessed via MTT assay. In addition, the Annexin-V-FITC/propidium iodide double labeling kit was used to investigate if PEG2000-ASP@Se could induce apoptotic cell death with flow cytometry. The generation of ROS had been shown to cause cell apoptosis and drug-induced cytotoxicity. Therefore, the intracellular levels of ROS in PEG2000-ASP@Se treated hMSCs were measured by using dichlorofl uorescein diacetate (DCFH-DA). Alizarin Red staining results suggested that PEG2000-ASP@Se nanoparticles can enhance the osteogenic differentiation of MSC. These results showed that the PEG2000-ASP@Se is a nano-drug with good cell compatibility.关键词
硒纳米粒子/间充质干细胞/成骨分化Key words
Se-nanoparticles/mesenchymal stem cells/osteogenic differentiation分类
医药卫生引用本文复制引用
郑楚萍,陶涛,张依,李凌剑,季红,陈法江..天冬氨酸修饰的纳米硒 (PEG2000-ASP@Se) 对间充质干细胞增殖与分化能力的影响[J].安徽农业大学学报,2018,45(6):1107-1111,5.基金项目
广东省创新训练项目(201710570096) （201710570096）
广州市教育科学规划课题(201811666) （201811666）
广州医科大学大学生科技创新项目(2017A001)共同资助 （2017A001）
