安徽农业大学学报2018，Vol.45Issue(6)：1112-1118,7.DOI:10.13610/j.cnki.1672-352x.20190102.013
杜仲内生细菌对植物病原菌的拮抗作用
Antagonistic effect of endophytic bacterial strain isolated from Eucommia ulmoides on phytopathogen
摘要
Abstract
To investigate the biological control agents to counteract the maize disease, plate dilution method was used to isolate the strains from the healthy Eucommia ulmoides. The results showed that the total of 55 strains of endophytic bacteria in Eucommia ulmoides were obtained, belonging to 5 genera: Bacillus, Paenibacillus, Burkholderia, Cupriavidus and Lysinibacillus. Then the antimicrobial effects of the endophytic bacteria were tested by dual culture, and the candidate DZSY21 was found to have significantly antagonistic effects on the growth and development of Bipolaris maydis and Fusarium moniliforme, resulting in the inhibitory rates of 61.30％ and 79.10％, respectively. Microscopic examination of affected mycelia of phytopathogen showed some mycelia treated with the DZSY21 were swelled, contorted and broken. Molecular characterization assays indicated that the DZSY21 is Bacillus subtilis, containing chemotaxis to the root exudates of maize.关键词
杜仲/内生细菌/分离/拮抗作用Key words
Eucommia ulmoides/endophytic bacteria/isolation/antagonism分类
农业科技引用本文复制引用
苏博,姚沛琳,刘小阳,姜华,王明智,丁婷..杜仲内生细菌对植物病原菌的拮抗作用[J].安徽农业大学学报,2018,45(6):1112-1118,7.基金项目
国家重点研发计划课题植物微生态制剂的研制与应用(2017YFD0201106) （2017YFD0201106）
宿州学院科研开放平台(2017ykf01) （2017ykf01）
宿州学院科研开放平台(2016ykf11)共同资助 （2016ykf11）
