安徽农业科学2019，Vol.47Issue(2)：80-82,3.DOI:10.3969/j.issn.0517-6611.2019.02.023
亚硒酸钠对小鼠脾脏淋巴细胞体外增殖及细胞因子分泌的影响
Effects of Sodium Selenite on the Proliferation of Splenic Lymphocytes and Cytokine Secretion in vitro of Mice
摘要
Abstract
[Objective]To study the effects of sodium selenite on the spleen lymphocyte's proliferation and the cytokines secretion of mice in vitro, and explore the possible immune regulation ways of sodium selenite.[Method]The lymphocyte was isolated from the spleen of BALB/c mice.MTS method was used to detect the proliferation rate of lymphocytes cultured in sodium selenite medium with different concentrations (0, 10-8, 10-7, 10-6 and 10-5 mol/L) after 48 h and screen out the optimum concentration of sodium selenite for spleen lymphocyte culture.The secretion quantity of IFN-γ, IL-1β, IL-4 and IL-6 in culture supernatant after culturing 48 h were measured by ELISA.[Result]The proliferation rate of lymphocytes treated by 10-7 mol/mL sodium selenite was significantly increased compared with that in control group, and the secretion quantity of IFN-γ, IL-1β, IL-4 and IL-6 in culture supernatant increased by 10.34, 2.15, 1.06, and 6.68 times, respectively.[Conclusion]Sodium selenite may regulate the body immunity by regulating spleen lymphocyte's proliferation and secretion of cytokines.关键词
亚硒酸钠/小鼠/淋巴细胞/增殖率/细胞因子Key words
Sodium selenite/Mice/Lymphocyte/Proliferation rate/Cytokine分类
农业科技引用本文复制引用
李冰心,李婉雁,田允波,曹楠,许丹宁..亚硒酸钠对小鼠脾脏淋巴细胞体外增殖及细胞因子分泌的影响[J].安徽农业科学,2019,47(2):80-82,3.基金项目
广东省"创新强校工程"基础研究重大项目(2017KZDXM046) （2017KZDXM046）
广东省科技计划项目(2017B020202010) （2017B020202010）
