安徽农业科学2019，Vol.47Issue(2)：156-158,3.DOI:10.3969/j.issn.0517-6611.2019.02.047
植物乳杆菌LPL10的体外益生特性研究
Vitro Probiotic Properties of Lactobacillus plantarum LPL10
摘要
Abstract
[Objective]The probiotic characteristics of Lactobacillus plantarum LPL10 obtained from food products were evaluated through acid tolerance, bile salt resistance, antibacterial activity and acid-producing ability. [Method]The resistance to acid and bile salt of LPL10 were researched by plate counting method, its antibacterial property by the inhibition zone method, the acid-producing ability by high performance liquid chromatography.[Result]Lactobacillus plantarum LPL10 had a good acid-lowering tolerance, and the viable count was 1.2×108 cfu/mL in 24 h at pH 3.5, the survival rate was 148％. There was low suppression on containing 0.1％ bile salt for LPL10, the viable count was 3.60×108 cfu/mL after the culture of 24 h and its survival rate was 356％; LPL10 played an inhibitory role in Staphylococcus aureus, Micrococcus luteus, and Pseudomonas aeruginosa.Lactic acid and acetic acid were the main organic acids produced by fermenlatin broth of LPL10, the relative contents were 20.33 and 25.26 mg/L, respectively. [Conclusion]The research results showed that Lactobacillus plantarum LPL10 could be a potential probiotic strain.关键词
植物乳杆菌/耐酸/耐胆盐/抑菌/产酸能力Key words
Lactobacillus plantarum/Acid tolerance/Bile salt resistance/Antibacterial activity/Acid-producing ability分类
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陈琼,甘祥武,黄魁英,赵乐怡,李学优,鲍志宁,曹丁,邓钧尹..植物乳杆菌LPL10的体外益生特性研究[J].安徽农业科学,2019,47(2):156-158,3.基金项目
2018年广东省微生物种质资源库项目 （）
