安徽医科大学学报2019，Vol.54Issue(1)：15-20,6.DOI:10.19405/j.cnki.issn1000-1492.2019.01.004
鸡豆黄素A对围绝经期抑郁症大鼠的神经保护作用及机制研究
Research on effects and mechanism of Bioch A neuroprotection to rats in perimenopausal depression disorder
摘要
Abstract
Objective To explore behavioral influence and neuroprotective mechanism of Bioch A in inperimenopausal depression disorder (PDD) rats model. Methods Rats model of PDD was established via two-step methods ovariectomy (OVX) and chronic unpre-dictable mild stress (CUMS).Rats'behavioral changes were observed through open-field tests and forced-swimming tests;a HPLC method with electrochemical detector is applied for determination of dopamine (DA), arterenol (NA) and 5-hydroxytryptamine (5-HT) content in rats;Western blot was used to detecting expression of interleukin-1β (IL-1β), interleukin-1 converting enzyme (Caspase-1), protein kinase A (PKA), phosphorylation protein kinase A (p-PKA), cAMP response element binding pro-tein (CREB), phosphorylation cAMP response element binding pro-tein (p-CREB), brain-derived neurotrophic factor (BDNF), and tyrosine protein kinase B (TrkB).Results After Bioch A and fluoxetine treatment, rats in PDD scored obviously higher at both horizontal motion and vertical motion;immobility time in forced swimming was significantly shortened (P＜0.05, P＜0.01);expression level of IL-1βand Caspase-1 lowered in different degrees (P＜0.05, P＜0.01);5-HT content was clearly increased;phosphorylation level of PKA and CREB, expression level of BD-NF, Trk B and mRNA all rose in different degrees (P＜0.05, P＜0.01), in comparison with those of model group. Conclusion Bioch A has a certain antagonistic effect on PDD, and its mechanism is mainly related to increasing hippocampal 5-HT content and up-regulating hippocampal cAMP-CREB-BDNF signaling pathway protein expression.关键词
鸡豆黄素A/围绝经期抑郁症/蛋白激酶A/cAMP反应元件结合蛋白/脑源性神经营养因子/酪氨酸蛋白激酶BKey words
biochanin A/perimenopausal depression/protein kinase A/cAMP response element binding protein/brain derived neurotrophic factor/tyrosine protein kinase B分类
医药卫生引用本文复制引用
杨琴,梁枫,李正姐,贺志鹏,黄超娟,丁楚涵,尹艳艳..鸡豆黄素A对围绝经期抑郁症大鼠的神经保护作用及机制研究[J].安徽医科大学学报,2019,54(1):15-20,6.基金项目
安徽省自然科学基金项目(编号:1508085MH186) （编号:1508085MH186）
安徽高校自然科学研究项目(编号:KJ2018A0796) （编号:KJ2018A0796）
大学生创新创业训练计划(编号:201610366065) （编号:201610366065）
安徽中医药高等专科学校自然科学研究项目(编号:ZRKX1802) （编号:ZRKX1802）
