安徽医科大学学报2019，Vol.54Issue(1)：27-32,6.DOI:10.19405/j.cnki.issn1000-1492.2019.01.006
ARAP3-VAV2新结合方式鉴定与功能分析
Identification and functional analysis of new ARAP3 and VAV2 interaction model
摘要
Abstract
Objective To study the new interaction model between adenosine diphosphate ribosylation factor 6guanosine triphosphatase activating protein 3 (ARAP3) and VAV guanine nucleotide exchange factor 2 (VAV2), further to analyze their possible physiological functions. Methods Eukaryotic expression wild type and mutantion plasmids of ARAP3 and VAV2 were constructed respectively.The co-immunoprecipitation assays with transfected human cervical carcinoma cells (HeLa cells) and human embryonic kidney cells (HEK-293T cells) were used to detect the interaction model between ARAP3 and VAV2.The fluorescence labeling experiments were used to test the effect of overexpression of ARAP3, VAV2 and their mutants on fibrous actin (F-actin) assembly.ResultsN-terminal region of VAV2 (1-660aa) could also mediate its interaction with ARAP3 besides sarcoma gene homology 2 (SH2) domain of VAV2.Overexpression of VAV2 and its mutants in HeLa cell respectively could regulate the F-actin organization as same as ARAP3. Conclusion Several domains of VAV2 mediate its interaction with ARAP3, which may promote the dynamic changes of rat sarcoma homologus oncogene A (RhoA) activity and F-actin assembly, even cell migration and cell invasion.关键词
ARAP3/VAV2/磷酸化/F-actinKey words
ARAP3/VAV2/phosphorylation/F-actin分类
生物科学引用本文复制引用
徐光生,许文娟,宋雪燕,王峰松..ARAP3-VAV2新结合方式鉴定与功能分析[J].安徽医科大学学报,2019,54(1):27-32,6.基金项目
国家自然科学基金面上项目(编号:31271518) （编号:31271518）
