安徽医科大学学报2019，Vol.54Issue(1)：90-96,7.DOI:10.19405/j.cnki.issn1000-1492.2019.01.018
miR-133b对喉癌细胞增殖、凋亡、侵袭的影响及机制研究
Effects of miR-133b on proliferation, apoptosis and invasion of laryngeal carcinoma cells and its mechanism
摘要
Abstract
Objective To explore the effects and mechanism on proliferation, apoptosis and invasion behavior of laryngeal carcinoma Hep-2 cells by miR-133b through GSTP1. Methods Based on high throughput data set GSE47610 of miRNA chip of laryngeal carcinoma from Gene Expression Omnibus (GEO), differentially expressed miRNAs were obtained by bioinformatics analysis using Qlucore Omics Explorer (QOE) 3.2.Real-time quantitative PCR technique was used to detect the relative expression of miR-133b in 46 patients with laryngeal carcinoma.The clinical characteristics of miR-133b relative expression were analyzed.hsa-miR-133b was transfected to Hep-2 cells and the transfection efficiency was detected through fluorescence microscopy.For overexpression of miR-133b group and NC group Hep-2 cells, cell proliferation, cell cycle and apoptosis, cell invasion were measured by MTT method, flow cell technology and Tanswell respectively.miR-133b target gene was verified by bioinformatics prediction and luciferase reporter gene experiment, and target gene GSTP1 gene protein expression was detected by Western blot.Results 5 up-regulated expression miRNAs and 8 down-regulated expression miRNAs in laryngeal carcinoma were obtained and miR-133b was selected for functional study.miR-133b expression significantly lowered about4.1 times in laryngeal carcinoma tissue sample, and the expression of miR-133b levels negatively correlated with local tumor infiltration depth, lymph node metastasis, clinical stage and histological grade.Transfected hsa-miR-133b mimic to laryngeal carcinoma Hep-2 cells and the transfection efficiency was over 80％, overexpression miR-133b in Hep-2 cells was achieved.Overexpression of miR-133b can significantly inhibit the laryngeal carcinoma cell proliferation, affect cell cycle, promote cell apoptosis and inhibit cell migration.It was clear that miR-133b targeted with GSTP1, and overexpression of miR-133b can significantly reduce the expression of GSTP1 protein. Conclusion miR-133b can regulate laryngeal carcinoma Hep-2 cell proliferation, apoptosis, invasion behavior by targeted GSTP1, and hsa a certain clinical significance, which provide new ideas and theoretical basis of clinical diagnosis, prognosis and molecular targeted therapy of laryngeal carcinoma in the future.关键词
miR-133b/GSTP1/喉癌Hep-2细胞/增殖/凋亡/侵袭Key words
miR-133b/GSTP1/laryngeal cancer Hep-2 cells/proliferation/apoptosis/migration分类
医药卫生引用本文复制引用
郭有新,陈宝刚,刘建伟,李立,张红,马志红..miR-133b对喉癌细胞增殖、凋亡、侵袭的影响及机制研究[J].安徽医科大学学报,2019,54(1):90-96,7.基金项目
国家自然科学基金(编号:81502493) （编号:81502493）
