安徽医科大学学报2019，Vol.54Issue(2)：203-206,4.DOI:10.19405/j.cnki.issn1000-1492.2019.02.008
18 F-氟赤硝基咪唑micro PET/CT评价裸鼠乳腺癌早期放疗疗效实验研究
Experimental study on the efficacy of early radiotherapy for breast cancer in nude mice by 18 F-FETNIM micro PET/CT
摘要
Abstract
Objective To investigate the value of 18F-FETNIM micro PET /CT in assessing the radiotherapeutic efficacy in MDA-MB231 breast cancer model. Methods Sixteen MDA-MB231 breast cancer models were randomly diveded into two groups(8 rats in each group):control group(group A) and radiotherapy group(group B). 18 FFETNIM micro PET /CT imaging was performed in two groups to measure the SUVmax of tumor in all nude mice. The morphological features of tumor tissue in each group were observed by HE staining. The expression of hypoxia inducible factor 1α(HIF-1α) in two groups were determined by immunohistochemistry. Results There was no significant difference in the SUVmax value between control group and radiotherapy group before radiotherapy (t = 0. 375,P > 0. 05). The SUVmax value of tumor tissue when 48 h after radiotherapy was significantly lower than that before radiotherapy (t = 9. 958,P < 0. 05) and 24 h after radiotherapy(t = 16. 506,P < 0. 05),the difference was statistically significant(F = 58. 860,P < 0. 05). The SUVmax value of tumor tissue when 48 h after radiotherapy was also lower than before radiotherapy(t = 5. 405,P < 0. 05). More obvious necrosis was observed in the radiotherapy group than the control group by HE staining. The expressions of HIF-1α in the radiotherapy group were significantly decreased compared with the control group(t = 14. 802,P < 0. 05). The tumor SUVmax value was positively correlated with HIF-1α expression(r = 0. 865,P < 0. 05). Conclusion 18F-FETNIM micro PET /CT not only can be used to detect the hypoxic state within the tumor,but also can evaluate the early radiotherapeutic efficacy in MDA-MB231 breast cancer in nude mice.关键词
18 F-氟赤硝基咪唑/micro PET/CT/放疗疗效/乏氧诱导因子-1αKey words
18F-FETNIM/micro PET /CT/radiotherapeutic efficacy/HIF-1α分类
医药卫生引用本文复制引用
苏晓雨,徐慧琴,汪会,余文静,张丹,谯凤..18 F-氟赤硝基咪唑micro PET/CT评价裸鼠乳腺癌早期放疗疗效实验研究[J].安徽医科大学学报,2019,54(2):203-206,4.基金项目
安徽省科技厅科技攻关项目(编号:1704a0802164) （编号:1704a0802164）
