安徽医科大学学报2019，Vol.54Issue(2)：212-216,5.DOI:10.19405/j.cnki.issn1000-1492.2019.02.010
lncRNA NEAT1在结核分枝杆菌感染RAW264.7巨噬细胞中的表达及作用的初步探讨
The expression and function of lncRNA NEAT1 in RAW264. 7 macrophages infected with Mycobacterium tuberculosis
摘要
Abstract
Objective To detect the expression changes of lncRNA NEAT1 in RAW264. 7 macrophages after infected with tuberculosis. By lowering the expression of NEAT1 in cells,the role of its in anti-tuberculosis immune response in macrophages was preliminarily discussed. Methods RAW264. 7 macrophages were infected with H37Rv,cells and culture supernatant were collected in the 12 h,24 h,48 h time points. Respectively,RT-qPCR was used to detect intracellular NEAT1 expression,and ELISA assay was used to detect IL-6 expression in culture supernatant. By using RNAi,NEAT1 was silenced in macrophages,and after H37Rv infection,the secretion of was detected,and the change of bactericidal capacity agasint MTB was assessed. Results Test results showed that the expression of NEAT1 and secretion of IL-6 were both significantly increased (P < 0. 01). By silencing NEAT1 in macrophages,there was a significant decrease in secretion of IL-6 (P < 0. 01),and the ability of MTB removal was significantly decreased (P < 0. 01). Conclusion H37Rv infection RAW264. 7 macrophages can promote NEAT1 expression,silencing NEAT1 can reduce the clearance of intracellular MTB by macrophages.关键词
结核分枝杆菌/lncRNA NEAT1/RAW264. 7 巨噬细胞Key words
Mycobacterium tuberculosis/lncRNA NEAT1/RAW264. 7 macrophages分类
医药卫生引用本文复制引用
黄舒颖,张诚,黄自坤,罗清,卿城..lncRNA NEAT1在结核分枝杆菌感染RAW264.7巨噬细胞中的表达及作用的初步探讨[J].安徽医科大学学报,2019,54(2):212-216,5.基金项目
江西省自然科学基金(编号: 20171BAB205028、20161BAB215224) （编号: 20171BAB205028、20161BAB215224）
江西省教育厅科学技术研究项目(编号:GJJ160120) （编号:GJJ160120）
江西省卫生计生委科技计划项目(编号:20185084) （编号:20185084）
