安徽医科大学学报2019，Vol.54Issue(2)：220-224,5.DOI:10.19405/j.cnki.issn1000-1492.2019.02.012
二氢杨梅素对SHED活性及其成骨分化能力的影响
Effects of Dihydromyricetin on the activity and osteogenic differentiation of stem cells from human exfoliated deciduous teeth
摘要
Abstract
Objective To observe the effect of Dihydromyricetin(DMY) on activity and osteogenic differentiation of stem cells from human exfoliated deciduous teeth(SHED) in vitro. Methods SHED were gained by Enzymatic digestion in vitro. The cellular viability,alkaline phosphotase activity,osteogenic differentiation related genes ,such as Runt-related transcription factor 2(Runx2) and osteocalcin (OCN) were observed after different concentration of DMY act upon SHED. Results 0. 02,0. 1,0. 5,2,10 and 50 μmol /L concentration of DMY had no cytotoxicity to SHED. 10,50 μmol /L concentration of DMY increased the activity of alkaline phosphatase(P < 0. 05). 0. 1,0. 5, 2,10 and 50 μmol /L concentration of DMY increased the mRNA expressions of Runx2,0. 5,2,10 and 50 μmol /L concentration of DMY increased the mRNA expressions of OCN which were in a concentration-dependent manner(P< 0. 05). Conclusion 0. 02,0. 1,0. 5,2,10,50 μmol /L concentration have no significant effect on the activity of SHED. A certain concentration of DMY can enhance the osteogenic differentiation of SHED.关键词
二氢杨梅素/人乳牙牙髓干细胞/细胞活性/成骨分化Key words
Dihydromyricetin/SHED/cell activity/osteogenic differentiation分类
医药卫生引用本文复制引用
岳二丽,李夏宁,赵红宇,王一飞,刘飞,肖剑礴..二氢杨梅素对SHED活性及其成骨分化能力的影响[J].安徽医科大学学报,2019,54(2):220-224,5.基金项目
河南省高等学校重点科研项目计划(编号:17A320012) （编号:17A320012）
河南省医学科技攻关计划项目(编号:201602015) （编号:201602015）
