安徽医科大学学报2019，Vol.54Issue(2)：231-236,6.DOI:10.19405/j.cnki.issn1000-1492.2019.02.014
HK2在肝癌中的差异表达及其对肝癌细胞的影响
Differential expression of HK2 in HCC and its effect on HCC cells
摘要
Abstract
Objective To investigate the differential expression of HK2 in liver cancer tissues and its effects on cell proliferation ,cell cycle and apoptosis of liver cancer cells . Methods 48 cases of liver cancer tissues and corresponding para cancer tissues were collected,and the expression of HK2 was detected by immunohistochemistry. At the same time,Western blot was used to detect the expression of HK2 in human hepatoma cell HepG2 and in normal liver cell L-02. The relationship between HK2 expression and clinic pathological characteristics of hepatocellular carcinoma was analyzed statistically. Four HK2 shRNA vectors were constructed,Western blot was used to detect the interference efficiency,and the best HK2 shRNA was selected for transfecting cell. For blank group (normal culture,non-transfected plasmid),control shRNA group(transfected control shRNA),HK2 shRNA group (transfected shRNA_3) HepG2 cells,MTT was used to detect cell proliferation activity,flow cytometry was used for cell cycle,Annexin V-FITC /PI double labeling method was used to detect cell apoptosis. Results The expression of HK2 increased significantly in HCC tissues,and the expression was correlated with tumor diameter,TNM stage and histopathological grade. The decrease of HK2 expressionsignificantly reduced the proliferation activity of HepG2 cells,obviously changed the cell cycle and make cells less stagnant at S stage,significantly increased the apoptosis of HepG2 cells. Conclusion HK2 shows strong anti-tumor potential and has a certain clinical significance. It provides a new idea and theoretical basis for the clinical diagnosis of liver cancer,prognosis judgment and molecular targeting therapy based on HK2.关键词
HK2/肝细胞癌/shRNA 沉默Key words
HK2/hepatocellular carcinoma/shRNA silence分类
医药卫生引用本文复制引用
张宇,蒙轩,王勋..HK2在肝癌中的差异表达及其对肝癌细胞的影响[J].安徽医科大学学报,2019,54(2):231-236,6.基金项目
国家自然科学基金(编号:81502376) （编号:81502376）
北京市自然科学基金面上项目(编号:7172201) （编号:7172201）
北京市科技新星计划(编号:Z181100006218089) （编号:Z181100006218089）
