安徽医科大学学报2019，Vol.54Issue(2)：251-255,5.DOI:10.19405/j.cnki.issn1000-1492.2019.02.018
钛纳米管表面固定GL13K抗菌肽的实验研究
The experimental study of immobilization of the GL13K antimicrobial peptide on the TiO2 nanotubes in vitro
摘要
Abstract
Objective To investigate the method of immobilizing GL13K antibacterial peptide(AMP) on the surface of titanium dioxide(TiO2) nanotubes and compare the effects thereof. Finallly,offering new ideas for antimicrobial modification of titanium implant surface and the treatment of peri-implantitis. Methods The GL13K AMP was immobileized on the TiO2 nanotubes by physical adsorption,covalently bonding and layer-by-layer self-assembly to construct a bioactive antibacterial coating,and the fixation effect was compared. Results We characterized the sGL13Kles by field emission scanning electron microscopy,fluorescence microscopy,contact angle measureing instrument,X-ray photoelectron spectroscopy,Fourier infrared spectroscopyand ultraviolet spectrophotometer. It was confirmed that all three methods can fix GL13K AMP on the TiO2 nanotubes. Conclusion Layer-by-layer selfassembly method has the best sustained release effect and it is expected to improve the sust-ained antibacterial properties of the titanium implant surface.关键词
TiO2纳米管/GL13K 抗菌肽/缓释/种植体周围炎Key words
TiO2 nanotubes/GL13K antimicrobial peptide/controlled-release/peri-implantitis分类
医药卫生引用本文复制引用
王泽华,程佳蕙,高啟坤,宋婷婷,吴明月..钛纳米管表面固定GL13K抗菌肽的实验研究[J].安徽医科大学学报,2019,54(2):251-255,5.基金项目
国家自然科学基金(编号:81170993) （编号:81170993）
