安徽医科大学学报2019，Vol.54Issue(2)：329-332,4.DOI:10.19405/j.cnki.issn1000-1492.2019.02.035
耐药结核分枝杆菌与耐药基因突变的相关性分析
Correlation analysis between drug-resistant Mycobacterium tuberculosis and drug-resistant gene mutation
摘要
Abstract
To understand the drug resistance and gene mutation of Mycobacterium tuberculosis(MTB) in patients with pulmonary tuberculosis. The resistance of Mycobacterium tuberculosis to Rifampin(RFP),isoniazid(INH), streptomycin(SM),pyrazinamide(PZA) and ethambutol(EMB) was detected by Roche susceptibility test in 68 clinical isolates. The polymerase chain reaction single strand conformation multistate analysis (PCR-SSCP) was used to detect tuberculosis branching. Bacterial rpoB,katG,rpsL,pncA and embB gene mutations. The mutation rates of rifampin resistant(rpoB),isoniazid(katG),streptomycin(rpsL),azinamine(pncA) and ethambutol(embB) were 78. 2％,69. 8％,72. 2％,43. 8％ and 31. 4％,respectively. The mutation rates of high resistant strains were 92. 1％,80. 0％,87. 5％,72. 0％ and 45. 5％,respectively. The low resistant plants were 47. 1％,25. 0％, 28. 5％,13. 0％ and 7. 7％,respectively. The mutation of drug-resistant gene of Mycobacterium tuberculosis is closely related to the level of drug resistance. The PCR-SSCP method is rapid,sensitive and specific for detecting rpoB, katG,rpsL,pncA and embB mutations in Mycobacterium tuberculosis.关键词
结核分枝杆菌/药敏实验/聚合酶链反应- 单链构像多态性Key words
Mycobacterium tuberculosis/drug sensitivity test/polymerase chain reaction-single strand conformational polymorphism分类
医药卫生引用本文复制引用
孔伟伟,邢应如,胡万发,胡东,吴璇,于莉,汪旻旻,黄升海..耐药结核分枝杆菌与耐药基因突变的相关性分析[J].安徽医科大学学报,2019,54(2):329-332,4.基金项目
国家自然科学基金(编号:81371797、81571528) （编号:81371797、81571528）
淮南市科技计划项目(编号:2015A2401、2017A0592) （编号:2015A2401、2017A0592）
