北京中医药大学学报2019，Vol.42Issue(1)：21-29,9.DOI:10.3969/j.issn.1006-2157.2019.01.005
玄参提取物对甲亢模型大鼠肝脏代谢组学的影响
Impact of extracts of Xuanshenon liver metabolome in rat modelof hyperthyroidism
摘要
Abstract
Objective To investigate the effect and possible mechanism of extracts of Xuanshen (Scrophularia Root, Radix Scrophulariae) on liver metabolome in rat model of hyperthyroidism.Methods The rat liver metabolites spectrums effected by Euthyrox and Xuanshen extracts were analyzed by using ultra high performance liquid chromatography-time of-flight-mass spectrometry (UPLC-TOF-MS).The difference in metabolites spectrums was studied between blank group and model group by using orthogonal partial least squares-discriminant analysis (OPLS-DA).The potential biomarkers were found in liver through variable importance projection (VIP).The interventional effects of Xuanshen extracts were discussed through comparing the difference in content of potential biomarkers.Results There were 21 potential biomarkers found in rat liver in model group, and among them14 metabolites were significantly called back by Xuanshen extracts.The biosynthesis of glycosylphosphatidylinositol (GPI) -anchor, and metabolisms of arachidonic acid, purine, α-linolenic acid, linoleic acid, glutathione and glycerophospholipid were significantly improved by Xuanshen extracts in Euthyrox-induced hyperthyroidism rat model.Conclusion Xuanshen extracts can play a role of treating hyperthyroidism through improving relevant liver potential biomarkers and metabolism pathways in hyperthyroidism rat model.关键词
玄参/优甲乐/甲状腺功能亢进症/肝脏代谢组学/大鼠Key words
Xuanshen (Scrophularia Root, Radix Scrophulariae)/Euthyrox/hyperthyroidism/liver metabonomics/rats分类
医药卫生引用本文复制引用
张宁,李自辉,赵洪伟,庞牧,叶涛,刘树民..玄参提取物对甲亢模型大鼠肝脏代谢组学的影响[J].北京中医药大学学报,2019,42(1):21-29,9.基金项目
国家重点基础研究发展计划(973计划)资助项目(No.2013CB531804) （973计划）
国家自然科学基金青年科学基金资助项目(No.81302898) （No.81302898）
黑龙江省青年科学基金资助项目(No.QC2012C038) （No.QC2012C038）
黑龙江省教育厅科学技术研究项目(No.12541744) （No.12541744）
黑龙江中医药大学科研基金资助项目(No.B201104) （No.B201104）
