北京中医药大学学报2019，Vol.42Issue(1)：37-43,7.DOI:10.3969/j.issn.1006-2157.2019.01.007
麦门冬汤对肺纤维化大鼠肺功能及内质网应激作用的影响
Influence of MaimendongTang on lung function and endoplasmic reticulum stress in rats with pulmonary fibrosis
摘要
Abstract
Objective To investigate the relative mechanism of Maimendong Tang (Ophiopogon Decoction) in amelioration of lung function and endoplasmic reticulum stress (ERS) in rats with bleomycin-induced pulmonary fibrosis.Methods Male healthy SD rats were randomly divided into normal group, model group, Maimendong Tang group 1 and Maimendong Tang group 2.The rat model of pulmonary fibrosis was established by bleomycin pulverization via tracheal intubation.On the 14 t h d after modeling, Maimendong Tang was intragastrically given to Maimendong Tang group1 and Maimendong Tang group 2 twice a day for 20 d continuously.The general conditions were observed, and pathological changes of lung tissue were observed after HE and Masson staining.The forced vital capacity (FVC) , 0.4 second rate (FEV0.4/FVC) , mass FVC, inspiratory resistance (Ri) , expiratory resistance (Re) and dynamic pulmonary compliance (Cydn) were detected by using spirometer.The expressions of glucose regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) protein in lung tissue were analyzed by using Western blotting assay and immunohistochemistry staining technique.Results Compared with normal group, FVC, FVC/lung mass and Cydn decreased, and 0.4 second rate increased in model group.Compared with model group, FVC increased and 0.4 second rate decreased and Cydn increased in Maimendong Tang group 1 and Maimendong Tang group 2, and mass FVC increased in Maimendong Tang group 2.Pathological sections showed that there were chronic inflammatory cell infiltration in consolidation zone of lung tissue and massive collagen deposition, GRP78 and CHOP with high expressions were mainly positioned in vesicular type Ⅱ alveolar epithelial cells (AECⅡs).The inflammation was relieved, collagen deposition was decreased, protein expressions of GRP78 and CHOP were reduced and in Maimendong Tang group 2.Conclusion Maimendong Tang can significantly improve lung function and reduce lung interstitial collagen deposition, which may be related to regulation of protein expressions of GRP78 and CHOP in AECⅡs in consolidation zone of lung tissue, relief of ERS, and recovery of normal AECⅡs function.关键词
麦门冬汤/内质网应激/Ⅱ型肺泡上皮细胞/葡萄糖调节蛋白78/C/EBP同源蛋白/大鼠Key words
Maimendong Tang (Ophiopogon Decoction)/endoplasmic reticulum stress/type Ⅱ alveolar epithelial cells/glucose regulated protein 78/C/EBP homologous protein/rats分类
医药卫生引用本文复制引用
申萌萌,南亚楠,唐磊,田如玉,朱曼,康荻,张瑞颖,牛建昭,李亚东..麦门冬汤对肺纤维化大鼠肺功能及内质网应激作用的影响[J].北京中医药大学学报,2019,42(1):37-43,7.基金项目
国家自然科学基金资助项目(No.81202787、No.81573716) （No.81202787、No.81573716）
高等学校学科创新引智计划(No.b07007) （No.b07007）
