北京中医药大学学报2019，Vol.42Issue(1)：52-57,6.DOI:10.3969/j.issn.1006-2157.2019.01.009
养阴益气合剂对肺纤维化大鼠肺泡上皮间质转化的干预机制研究
Intervention mechanism of Yangyin Yiqi Heji to alveolus epithel mesenchymal transition in rats with pulmonary fibrosis
摘要
Abstract
Objective To study the interventional effect of Yangyin Yiqi Heji (Yin-nourishing Qireplenishing Mixture) to alveolus epithelial-mesenchymal transition in rats with bleomycin-induced pulmonary fibrosis.Methods Wistar rats in SPF class (n=120) were randomly divided into normal group, model group, prednisone group and low-dose, mid-dose and high-dose Yangyin Yiqi Heji groups.The rat model of pulmonary fibrosis was copied by using intratracheal instillation of bleomycin, and all groups were orally given corresponding medicinals for 28 d after modeling for 1 d.The rats were executed respectively on the 14th d and 28th d to collect lung samples for detection of left lung pathological changes by using HE and Masson staining.The mRNA expressions ofα-smooth muscle actin (α-SMA) , E-cadherin, collagen I (Col I) and laminin (LN) were detected in rigth lung by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR).Results The mRNA expressions ofα-SMA, Col I and LN increased, and E-cadherin expressions decreased in model group compared with normal group (P<0.05).After treatment, the mRNA expressions ofα-SMA, Col I and LN decreased, and mRNA expression of E-cadherin increased in all treatment groups (P<0.05).On the 28 th d, mRNA expression of E-cadherin increased in high-dose Yangyin Yiqi Heji group compared with prednisone groups (P<0.05) , and mRNA expression of LN deecresed in high-dose, mid-dose and low-dose Yangyin Yiqi Heji groups (P<0.05).Conclusion Yangyin Yiqi Heji can inhibit the epithelial-mesenchymal transition in rats with bleomycin-induced pulmonary fibrosis, and the mechanism may be related to down-regulation of genes ofα-SMA, Col I and LN and up-regulation of E-cadherin gene.关键词
养阴益气合剂/肺纤维化/上皮间质转化/大鼠Key words
Yangyin Yiqi Heji (Yin-nourishing Qi-replenishing Mixture)/pulmonary fibrosis/epithelial-mesenchymal transition/rats分类
医药卫生引用本文复制引用
孟丽红,王洪,董环,顾潇枫,于小林,刘彧杉,辛大永,邱新萍,张晓梅..养阴益气合剂对肺纤维化大鼠肺泡上皮间质转化的干预机制研究[J].北京中医药大学学报,2019,42(1):52-57,6.基金项目
北京市自然科学基金资助项目(No.7172247) （No.7172247）
