吉林大学学报（医学版）2019，Vol.45Issue(1)：7-11,5.DOI:10.13481/j.1671-587x.20190102
蚓激酶对胃癌SGC7901细胞增殖抑制和凋亡诱导作用
Inhibitory effect of lumbrokinase on proliferation and induction on apoptosis of gastric cancer SGC7901 cells
摘要
Abstract
Objective:To investigate the regulatory effect of lumbrukinase (LBK) on the gastric cancer SGC7901cells, and to clarify its mechanism.Methods:The SGC7901cells in the logarithmic growth phase were selected and divided into control group and 2, 4, 8U·mL-1 LBK groups.MTT assay was used to detect the inhibitory rates of proliferation of SGC7901cells in various groups in vitro at different time (24, 48and 72h) .Cell scratch assay was used to detect the migration abilities of the SGC7901cells in vitro in various groups.Flow cytometry was used to determine the apoptotic rates of SGC7901cells and the pencentages of cells at different cell cycles in various groups.The expression levels of Bcl-2, Bax, and caspase-3in the SGC7901cells in various groups were detected by Western blotting method.Results:The results of MTT assay showed that compared with control group, the inhibitory rates of SGC7901cells in different doses of LBK groups after treated for 24, 48and 72hwere increased (P＜0.01) .The cell scratch assay results showed that compared with control group, the migration distances of SGC7901cells in4and 8U·mL-1 LBK groups were increased significantly (P＜0.01) .The flow cytometry results showed that compared with control group, the apoptotic rates of SGC7901cells in 4and 8U·mL-1 LBK groups were increased significantly (P＜0.01) ;the percentages of cells in G1and S phases were decreased (P＜0.01) ;the percentages of cells in G2phase were increased (P＜0.01) .The results of Western blotting method showed that compared with control group, the Bcl-2protein expression level in the SGC7901cells in 8U·mL-1 LBK group was decreased (P＜0.05) ;the Bax and caspase-3protein expression levels were increased (P＜0.05) .Conclusion:LBK can inhibit the proliferation and migration abilities of SGC7901cells in vitro and induce the apoptosis;its mechanism is achieved through the regulation of expression levels of Bcl-2, Bax, and caspase-3proteins.关键词
蚓激酶/SGC7901细胞/细胞划痕实验/细胞凋亡/细胞周期Key words
lumbrokinase/SGC7901 cells/cell scratch/apoptosis/cell cycle分类
医药卫生引用本文复制引用
刘丹,杨晓波,王颖,于丽红,石焱,张英杰,康万军..蚓激酶对胃癌SGC7901细胞增殖抑制和凋亡诱导作用[J].吉林大学学报（医学版）,2019,45(1):7-11,5.基金项目
国家自然科学基金青年基金项目资助课题(81502992) （81502992）
中国博士后科学基金资助课题(2015M572801) （2015M572801）
