吉林大学学报（医学版）2019，Vol.45Issue(1)：12-16,后插1,6.DOI:10.13481/j.1671-587x.20190103
BCG感染牛肺泡巨噬细胞的转录组测序和分析
Transcriptome sequencing and analysis of bovine alveolar macrophages infected with BCG
摘要
Abstract
Objective:To reveal the differentially expressed genes and long non-coding RNAs (lncRNAs) by sequencing the transcriptome of bovine alveolar macrophages (BAM) infected with Bacillus Calmette-Guérin (BCG) and analyzing their bioinformations, and to provide theoretical foundation for the research on immune regulation mechanism of anti-infection of Mycobacteriumtuberculosisof macrophages.Methods:The BAM were collected by pulmonary lavage and centrifugation and cultured and divided into infected group and uninfected group.After infection for 12hin infected group, the expression profiles of mRNA and lncRNA in infected group and uninfected group were detected by RNA-Seq, and bioinformatics analysis was carried out.Results:compared with uninfected group, there were 119differentially expressed lncRNAs and 1111differentially expressed mRNA in infected group (P＜0.05) .Gene Ontology functional enrichment analysis showed that the most significant enrichment was immune response (GO:0006955, P＜0.05) , including 125genes, in which 63 were up-regulated and 62 were downregulated, and the expressions of proinflammatory factors interleukin-1 (IL-6) , interleukin-7 (IL-7) , and interleukin-23A (IL-23A) were up-regulated.Cis target gene prediction and KEGG pathway analysis showed that the differentially expressed lncRNAs were involved in the regulation of transforming growth factor-β (TGF-β) signaling pathway and ATP-binding cassette transporter (ABC transporter) signaling pathway (P＜0.05) .Conclusion:The host cells are stimulated to produce a strong immune response after the BAM are infected by BCG and results in the changes of lncRNA and mRNA expression profiles.关键词
卡介苗/牛肺泡巨噬细胞/转录组测序/长链非编码RNAKey words
Bacillus Calmette-Guérin/bovine alveolar macrophage/transcriptome sequencing/long non-coding RNA分类
生物科学引用本文复制引用
徐金瑞,吕翠萍,杨易,周彦兵,骆佳,王玉炯..BCG感染牛肺泡巨噬细胞的转录组测序和分析[J].吉林大学学报（医学版）,2019,45(1):12-16,后插1,6.基金项目
国家自然科学基金资助课题(31572494,31760733) （31572494,31760733）
宁夏回族自治区科技厅自然科学基金资助课题(2018AAC03019) （2018AAC03019）
宁夏回族自治区重点研发计划项目资助课题(东西部合作,2017BN04) （东西部合作,2017BN04）
