吉林大学学报（医学版）2019，Vol.45Issue(1)：57-62,后插3,7.DOI:10.13481/j.1671-587x.20190111
菩人丹超微粉对糖尿病大鼠视网膜血流动力学和细胞因子表达的影响
Effects of Purendan Superfine Powder on retinal hemodynamics and expressions of cytokines in diabetic rats
摘要
Abstract
Objective:To investigate the effects of Purendan Superfine Powder (PRD) on the retinal hemodynamics and the expressions of cytokines in the diabetic rats, and to elucidate its protective effect on diabetic retinopathy (DR) .Methods:Thirty-six male SD rats were randomly divided into control group, diabetes group, and PRD group (n=12) .The diabetes rat models were established by intraperitoneal injecting streptozotocin (STZ) in diabetes group and PRD group.After successful modeling, the diabetic rats in PRD group were given PRD (1.8g·kg-1) by gavage for 3months.The hemodynamics parameters of central retinal artery (CRA) of the rats were measured with color Doppler ultrasound.The expression levels of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) mRNA in retina tissue were detected by reverse transcription polymerase chain reaction (RT-PCR) .Immunohistochemical staining and Western blotting methods were used to detect the expression levels of VEGF and bFGF proteins in retina tissue of the rats.Results:Compared with control group, the blood glucose, urine volume, liver index and kidney index of the rats in diabetes group were significantly increased (P＜0.05) , the peak systolic velocity (PSV) , end diastolic velocity (EDV) and mean velocity (MV) of CRA were significantly decreased (P＜0.05) , the resistant index (RI) and pulsatility index (PI) of CRA were significantly increased (P＜0.05) , and the expression levels of VEGF and bFGF mRNA and protein in retina tissue were significantly increased (P＜0.05) .Compared with diabetes group, the blood glucose, urine volume, liver index and kidney index of the rats in PRD group were significantly decreased (P＜0.05) , the PSV, EDV and MV of CRA were increased (P＜0.05) , the RI and PI of CRA were decreased (P＜0.05) , the expression levels of VEGF and bFGF mRNA and protein in retina tissue were significantly reduced (P＜0.05) .Conclusion:PRD can protect the diabetic retinal microvascular injury by reducing the blood glucose level, increasing the blood supply of retinal tissue, increasing the amount of microcirculation blood perfusion, reducing the vascular bed perfusion resistance, and reducing the expression of VEGF and bFGF.关键词
糖尿病, 实验性/糖尿病视网膜病变/菩人丹超微粉/血流动力学/血管内皮生长因子/碱性成纤维细胞生长因子Key words
diabetes mellitus, experiment/diabetic retinopathy/Purendan Superfine Powder/hemodynamics/vascular endothelial growth factor/basic fibroblast growth factor分类
医药卫生引用本文复制引用
王海彬,董志军,郭立涛,石晶,董薇丽..菩人丹超微粉对糖尿病大鼠视网膜血流动力学和细胞因子表达的影响[J].吉林大学学报（医学版）,2019,45(1):57-62,后插3,7.基金项目
河北省中医药管理局项目资助课题(2014065) （2014065）
